根癌农杆菌介导的紫花苜蓿遗传转化体系的建立与优化  

Establishment and Optimization of Agrobacterium-mediated Transformation of Alfalfa

作　　者：刘小琳[1] 王继峰[1] 胡晓艳[1] 刘艳昆[1] 熊军波[1] 康俊梅[1] 杨青川[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100094

出　　处：《中国草地学报》2007年第2期102-106,共5页Chinese Journal of Grassland

基　　金："十五"国家高技术发展计划(863计划)(2002AA241101);国家自然科学基金项目"紫花苜蓿盐胁迫特异表达基因的筛选与克隆"(30471110)

摘　　要：以“中苜1号”紫花苜蓿7日龄无菌苗子叶和再生无菌苗的叶片为材料,建立了适用于农杆菌介导的转基因组织培养体系,并对MsNHX1基因进行转化。转化优化条件为:“中苜1号”紫花苜蓿7日龄无菌苗子叶、再生无菌苗叶片,用农杆菌菌液(A600=0.6)侵染6min,然后在培养基上铺一层灭菌滤纸培养7d后清洗,建立了苜蓿快速有效的遗传转化体系。The regeneration system of somatic embryogenesis of genetic transformation for the cultivar alfalfa‘Zhongmu No. 1 ’ was established. Manipulation system was optimized by Kin--resistant calli assays and the histological detection of MsNHX1 activity, MsNHX1 gene corned from plant expression vector pBINHX. Two explants were infected with Agrobacterium tumefaciens （A600 = 0.6） for 8 rain and washed after 7 days of culture.

关 键 词：紫花苜蓿 遗传转化 根癌农杆菌 MsNHX1基因 

分 类 号：S541[农业科学—作物学]