资冲颗粒灌肠和灌胃对去势小鼠雌激素水平的影响  

Effect of Zichong Granules on estradiol level in ovariectomized mice administered by clyster and oral perfusion

作　　者：段恒[1] 陆华[2] 

机构地区：[1]重庆医科大学中医药学院中医临床教研室,重庆400050 [2]成都中医药大学临床医学院

出　　处：《北京中医药大学学报》2007年第3期185-187,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：四川省中医药管理局资助项目(No.2000-53)

摘　　要：目的探讨资冲颗粒灌肠和灌胃对去势小鼠雌激素水平及其效应的影响。方法对去势小鼠采用灌肠和灌胃两种方法给予资冲颗粒,并与己烯雌酚、女金丹进行对照。对小鼠阴道细胞角化指数、子宫指数进行计算;对子宫组织形态学进行观察;采用酶免疫分析法测定血清雌二醇(E2)水平。结果资冲颗粒灌肠组、资冲颗粒灌胃组、己烯雌酚组、女金丹组作用相似,均可增加去势小鼠阴道细胞角化指数(P<0.01)、子宫指数(P<0.01),提高小鼠血清E2水平(P<0.05),增加小鼠子宫内膜厚度(P<0.05)。结论资冲颗粒直肠给药和灌胃给药均可提高去势小鼠雌激素水平和产生雌激素样效应。提示资冲颗粒促卵泡发育的作用可能与提高雌激素水平有关。Objective To study the effect of the Zichong Granules （ZG） on estradiol （E2 ） level in the ovariectomized mice administered by different ways. Methods Ovariectomized mice were administered with ZG by ctyster or by oral perfusion. Diethylstilbestrol and Nüjindan were taken as control. Cornification index（CI） of vaginal cell and uterus index of castrated mice were counted. Histomorphology of uterus were observed. In addition, E2 in blood were determinated by enzyme immunoassay （EIA）. Results The effects of ZG administered by clyster or by oral perfusion, Diethylstilbestrol and Nüjindan were similar. They all increased cornification index （CI） of vaginal cell （P 〈 0.01 ） , uterus index （P 〈 0. 01 ）, E2 level in blood（ P 〈 0, 05 ） and the thickness of endometrium （P 〈 0.05 ） in the ovariectomized mice. Conclusion ZG administered by clyster or by oral perfusion has the effect of increasing E2 level and presents the estrogenlike effect. The effect of ZG for improving follicular development may be related to improving the level of E2.

关 键 词：资冲颗粒 去势小鼠 雌二醇 细胞角化指数 子宫指数 

分 类 号：R285.5[医药卫生—中药学]