RP-HPLC法测定Beagle犬血浆中清脑宣窍方有效部位4种主要成分的血药浓度  

Quantitative determination of 4 main components in effective fraction of Qingnaoxuanqiao Formula in beagle dogs plasma by RP-HPLC

作　　者：陈玉平[1] 石任兵[1] 刘斌[1] 孙建宁[1] 王永炎[2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国中医科学院

出　　处：《北京中医药大学学报》2007年第3期191-195,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：北京市教委产学研重点项目

摘　　要：目的建立血浆中清脑宣窍方有效部位中栀子苷、人参皂苷Rg1、Rb1及三七皂苷R1的HPLC血药浓度测定方法。方法血浆样品用C18固相柱萃取，以亥茅酚苷为内标，选用Agilent Eclipse XDB—C18色谱柱（4．6mm×250mm，5um），流动相为乙腈-水二元梯度洗脱，柱温为30℃；流速为1．0mL／min；检测波长为203nm。结果各成分的最低检测浓度（S／N〉3）在0．2～0．9mg／L之间，平均回收率均在90％以上，日内、日间精密度及稳定性的RSD均小于10％。结论该方法简便、准确。可作为清脑宣窍方血药浓度定量分析方法。Objective To establish a method for determinating the concentration of geniposide, ginsenoside Rg1 , ginsenoside RbI and notoginsenoside R1 in the effective fraction of Qingnaoxuanqiao Formula in beagle dog plasma by HPLC. Method Plasma samples were extracted by Cls solid phase column. Sec-o-glucosylhamaudol was taken as internal standard. Agilent Eclipse XDB-Clschromatography column （4.6 mm × 250 mm, 5 um） was applied. The gradient mobile phase was HCN-H2O system with column temperature 30 ℃ and flow rate 1.0 mL/min. The detecting wavelength was 203 nm. Results The limited concentrations of each component were between 0.2 -0.9 mg/L; the average recoveries were all above 90%; RSDs of intra- day and inter- days accuracies and stability were all below 10%. Conclusion The method is simple and accurate, and can be used as the quantitative analysis method for the 4 components in the effective fraction of Qingnaoxuanqiao Formula in plasma .

关 键 词：清脑宣窍方 栀子苷 人参皂苷Rg1 人参皂苷RB1 三七皂苷R1 高效液相色谱 含量测定 血药浓度 

分 类 号：R284.2[医药卫生—中药学]