色谱与X射线衍射技术在中成药鉴定中的联合应用  

Identifying Chinese tradition medicine by chromatograph and X-ray diffraction technology

作　　者：郑笑为[1] 龚宁波[2] 吴云山[2] 吕扬[2] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]中国医学科学院药物研究所,北京100050

出　　处：《药物分析杂志》2007年第3期315-318,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:将薄层色谱法与 X 射线衍射法联合用于黄连上清丸中成药的鉴定。方法:依据2000年版中国药典一部的薄层扫描含量测定方法,点于同一硅胶 G 薄层板上,以苯-乙酸乙酯-甲醇-异丙醇-水(20:10:5:5:1)为展开剂,氨蒸气饱和,进行荧光扫描,波长入=366nm,采用外标两点法测定计算;并采用 X 射线 Fourier 指纹图谱分析方法,分别对3个厂家的黄连上清丸样品进行测定,粉末 X-衍射的条件为 CuKα,管压40kV,管流150mA,扫描步长0.02°,扫描速度8°·min^(-1)。结果:获得了黄连上清丸中小檗碱的薄层扫描含量及黄连上清丸的 X 射线 Fourier 指纹图谱。结论:2种谱学方法的联合使用,可以获得中成药整体结构特征与药典指定的部分化学成分分析。Objective： To identify TCM Huanglianshangqing pill by thin - layer chromatography and powder X - ray diffraction. Method： Thin - layer chromatography scanning directed by Chinese Pharmacopoeia 2000, diffused by benzene - acetal - methanol - isopropyl alcohol - water （20：1 O： 5： 5： 1 ） mixed solvent, saturated by ammonia vapor,scanned at the wavelength 366 nm, calculated by outer two - point maker method ; The X - ray diffraction Fourier fingerprint pattern was adopted to determine Huanglianshangqing pill samples which come from three different manufactories. The X- ray experiment condition shown as： CuKα, tube voltage 40 kV, tube current 150 mA, scan step 0. 02°, scan speed 8°· min^-1. Results：Thin -layer chromatography scanning content of berberine in Huanglianshangqing pill was obtained. X - ray diffraction Fourier fingerprint pattern of Huanglianshangqing pill was also obtained. Conclusion：It is good that two methods was combined to achieve the whole structure character and to analyze partial chemical component specified in the Chinese Pharmacopoeia.

关 键 词：小檗碱 黄连上清丸 薄层扫描 X射线Fourier指纹图谱 

分 类 号：R917[医药卫生—药物分析学]