HPLC法测定双参龙胶囊中人参皂苷Rb_1的含量  

HPLC determination of ginsenoside Rb_1 in Shuangshenlong capsules

作　　者：李普衍[1] 韩晓萍[1] 

机构地区：[1]青海省药品检验所,西宁810000

出　　处：《药物分析杂志》2007年第3期429-430,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立双参龙胶囊中人参皂苷 Rb_1含量测定的高效液相色谱法。方法:采用 Kromasil C_(18)柱(KR100-5C_(18),4.6 mm×250 mm,5μm),乙腈-水(28.7:71.3)为流动相;流速为1.0 mL·min^(-1);检测波长为203 nm。结果:人参皂苷 Rb_1在0.848～4.24μg范围内,线性关系良好(r=0.9991),方法平均回收率为99.3%,RSD=1.2%(n=6)。结论:方法简便,结果准确,可用于双参龙胶囊中人参皂苷 Rb_1的定量。Objective： To develop a method for determination of ginsenoside Rb1 in Shuangshenlong capsules by HPLC. Method：The column was Kromasil C18 （ KR100 - 5 C18 ,4. 6 mm × 250 mm, 5 μm）. Acetonitrile - water （28.7： 71.3）was used as the mobile plase, the flow rate was 1.0 mL · min^-1 and detection wavelength was 203 nm. Results ：The linear range was between 0. 848 - 4. 24 μg for ginsenoside Rb1 （ r = 0. 9991 ）. The mean recovery was 99.3%, RSD = 1.2%. Conclusion：This method is found to be sensitive, quick and accurate for the determination of ginsenoside Rb1in Shuangshenlong capsules.

关 键 词：双参龙胶囊 人参皂苷RB1 含量测定 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]