离子对-反相高效液相色谱法测定人胎盘和羊水中利凡诺  

IP-RP-HPLC determination of rivanol in human placenta and amniotic fluid

作　　者：郭智勇[1] 魏丹毅[1] 储成顶[2] 尹耕心 贺明莉[3] 吴建力 傅克勤 

机构地区：[1]宁波大学材料科学与化学工程学院宁波市新型功能材料及其制备科学实验室-省部共建国家重点实验室培育基地,宁波315211 [2]安徽医科大学公共卫生学院,合肥230032 [3]安徽省计划生育研究所,合肥230031

出　　处：《药物分析杂志》2007年第3期457-459,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：安徽省人口和计划生育委员会专项基金;安徽省教育厅自然科学基金(2005KJ251);浙江省科技厅科技攻关项目(2006C33065)资助

摘　　要：目的:运用离子对-反相高效液相色谱法测定人胎盘和羊水中利凡诺的含量。方法:采用 Spherisorb C_(18)色谱柱(250mm×4.6 mm,5μm);柱温:30℃;流动相:甲醇-乙腈-0.05%十二烷基磺酸钠溶液(35:35:30,pH=3);流速:1.0 mL·min^(-1);荧光检测器:激发波长360 nm,发射波长505 nm。结果:人胎盘、羊水样品分别在10 ng·g^(-1)～1μg·g^(-1)、10 ng·mL^(-1)～1μg·mL^(-1)浓度范围内,均呈现良好线性,检测限分别为3 ng·g^(-1)、3 ng·mL^(-1),准确度和精密度好。结论:本方法操作简便,结果准确可靠。Objective： To establish an ion pair reverse - phase - high performance liquid chromatography （ IP - RP -HPLC） method for determining rivanol in human placenta and amniotic fluid. Method： Rivanol was chromatographed on a Spherisorb C18column （250 mm× 4.6 mm,5 μm） with the column temperature of 30 ℃. The mobile phase used was methanol -acetonitrile -0. 05 % sodium dodecylsulfonate （35：35：30, pH = 3 ）, with the flow rate at 1.0 mL · min^-1. Fluorimetric detector was used, with the excitation wavelength at 360 nm and the emission one 505 nm. Results：The standard curves of human placenta and amniotic fluid samples were linear in the range of 10 ng·g ^-1 μg·g and 10 ng·mL^-1-1 μg·mL^-1 eorrespondingly. The limit ofdeteetionwas3 ng g and 3 ng · mL^-1 respectively, and the accuracy and precision were good. Conclusion：The proposed method is simple, highly aeeurate and reliable,

关 键 词：反相高效液相色谱法 离子对 利凡诺 胎盘 羊水 

分 类 号：R917[医药卫生—药物分析学]