麦芽醇对大鼠局灶性脑缺血再灌注损伤后神经元凋亡的影响  

The Effects of Maltol on Neuronal Apoptosis after Focal Cerebral Ischemia-reperfusion in Rats

作　　者：高艳[1] 戴冀斌[2] 李应续[3] 

机构地区：[1]武汉大学基础医学院,湖北武汉430071 [2]武汉大学医学部解剖学教研室 [3]咸宁学院医学院解剖学教研室

出　　处：《咸宁学院学报（医学版）》2007年第1期4-6,共3页Journal of Xianning Univarsity(medical Sciences)

摘　　要：目的观察麦芽醇对大鼠局灶性脑缺血再灌注损伤后海马神经元内Caspase-3和神经元凋亡的影响。方法大脑中动脉线栓法建立脑缺血再灌注损伤模型,应用免疫印迹法和流式细胞计数法分别检测Caspase-3和细胞凋亡。结果再灌注72h后海马Caspase-3表达升高,凋亡细胞增多,麦芽醇组Caspase-3表达和凋亡细胞明显减少。结论麦芽醇对脑缺血再灌注损伤有保护作用。Objective To investigate the effects of maltol on expression of Caspase-3 protein in neurons and neuronal apoptosis after focal cerebral ischemia-reperfusion in rats. Methods A model of middle cerebral artery occlusion （ MCAO ） in rats was established with intraluminal filament occlusion. The Western-blot and flowcytomete were used respectively to observe the quantities of Caspase-3 protein and neuronal apoptosis in hippocampus. Results A significant increase in Caspase-3 immunoreactivity and apoptosis were detected at 72h after reperfusion. The difference of Caspase-3 and apoptosis between ischemic group and maltol group were significant. Conclusion Maltol can protect cerebral tissues by inhibiting cell apoptosis in ischemia reperfusin damages.

关 键 词：麦芽醇 脑缺血 凋亡 

分 类 号：R965.2[医药卫生—药理学]