大蒜多糖C对正常小鼠外周血淋巴细胞亚群的影响  

Effects of Garlic Polysaccharide C on Lymphocyte Subsets in the Peripheral Blood in Mice

作　　者：郑敏[1] 熊娟[2] 赵骥[3] 陈红光[3] 汪新亮[3] 

机构地区：[1]咸宁学院药学院药理学教研室,湖北咸宁437100 [2]华中科技大学同济医学院 [3]咸宁学院中心实验室

出　　处：《咸宁学院学报（医学版）》2007年第1期9-12,共4页Journal of Xianning Univarsity(medical Sciences)

基　　金：湖北省教育厅重点资助项目(2003A004);湖北省优秀青年基金项目(Q200628006)

摘　　要：目的研究大蒜多糖C对正常小鼠外周血淋巴细胞亚群的影响。方法30只昆明种小鼠随机分为正常对照组及大蒜多糖C组(800mg/kg ig,qd×12),应用流式细胞术检测小鼠外周血淋巴细胞亚群的百分比和CD4+/CD8+值。结果大蒜多糖C组小鼠外周血T、B、CD4+T淋巴细胞百分比较正常对照组均明显增加(P<0.05或P<0.01),CD8+及NK1.1+T淋巴细胞百分比无显著性变化(P>0.05),而CD4+/CD8+值显著高于正常对照组(P<0.05)。结论大蒜多糖C可使正常小鼠外周血T、B淋巴细胞含量增加,增强机体的细胞免疫及体液免疫功能。Objective To study the effects of garlic polysaccharide C on the peripheral-blood lymphocyte subsets in normal mice. Methods 30 kunming mice were randomly divided into two groups：normal control group and garlic polysaccharide C-treated group（800mg/kg ig,qd x 12）. The peripheral-blood lymphocyte subsets percentages and the CD4 ^＋/CD8^ ＋ ratio was measured by the flow cytometry. Results Compared with normal control group, the percentages of the T, B lymphocyte and the CD4^＋ T lymphocyte in the peripheralblood in garlic polysaccharide C-treated mice were siginificantly increased（ P 〈 0.05 or P 〈 0.01 ）, followed by the ratio of CD4^ ＋/CD8^＋ rising（P 〈 0.05）. However, the percentages of CD8^＋ and NK1.1^＋ T lymphocyte showed no obvious difference between the control and the treated groups（P 〉0.05 ）. Conclusion Garlic polysaccharide C can increase the percentages of the T, B lymphocyte in the peripheral blood in mice, and enhance cell immunity and humoral immune function.

关 键 词：大蒜多糖C 免疫 流式细胞术 淋巴细胞亚群 

分 类 号：R965.1[医药卫生—药理学]