迭鞘石斛抗肿瘤作用研究  

Inhibiting Effect of Tumor by Dendrobium denneanum

作　　者：罗傲雪[1] 宋关斌[1] 淳泽[2] 秦建[1] 罗傲霜[2] 范益军[3] 何涛[2] 

机构地区：[1]重庆大学生物工程学院,重庆400044 [2]中国科学院成都生物研究所,成都610041 [3]四川农业大学资源与环境系,四川都江堰611830

出　　处：《应用与环境生物学报》2007年第2期184-187,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家"十一五"支撑计划项目(2006BAI06A11-10);四川省"十一五"攻关计划项目(2006YZGG-12)~~

摘　　要：体外培养SMMC-7721和HL-7702细胞,以不同浓度的迭鞘石斛多糖、醇提物、水提物作为药物组,并设生理盐水组作为阴性对照,5-Fu组作为阳性对照,MTT法分别测定处理24h、48h、72h后的吸光度值,计算药物对细胞生长的抑制率.荷瘤小鼠分别腹腔注射迭鞘石斛3种提取物,连续注射10d,d11眼眶静脉取血,测定血清中MDA含量及SOD活力变化.体外实验发现浓度为160μg/mL的迭鞘石斛多糖作用48h,对SMMC-7721细胞的生长抑制作用最强,且对HL-7702细胞的毒害作用较小.体内实验发现,迭鞘石斛多糖能显著增强荷瘤小鼠血清中SOD活力(P<0.01),降低MDA含量(P<0.01).研究表明,迭鞘石斛多糖是一种具有潜力的抗肿瘤天然药物.This paper aims to study the anti-tumor activity and mechanism of Dendrobium denneanum in vitro and in vivo. In vitro, the inhibitory effects of D. denneanum on proliferation of human hepatoma SMMC-7721 cell and human natural liver HL- 7702 cell were measured by MTT colorimetric assay. The transplantable models of S180 in mice were established to investigate the inhibitory effects of the three extracts （ polysaccharide, ethanol extraction and water extraction） against tumor. The drug treatment lasted for 10 days, and the MDA and SOD contents of mouse blood serum were measured on d 11. In vitro, 160 μg/ mL polysaccharides were shown to have a prominent inhibition on SMMC-7721 for 48 hours, but an unapparent inhibition on HL-7702. In vivo, the SOD content of mouse blood serum in polysaccharide group was significantly higher than that of physiological salt solution group （ P 〈 0.01 ）. The MDA content of mouse blood serum in polysaccharide group was significantly lower than that of physiological salt solution group （ P 〈 0.01 ）. This study indicates that D. denneanum polysaccharide is a potential anti-tumor drug. Fig 1, Tab 2, Ref 8

关 键 词：迭鞘石斛 多糖 抗肿瘤作用 SMMC-7721 S180 

分 类 号：R285[医药卫生—中药学]