促红细胞生成素(EPO)对新生大鼠心肌细胞缺氧-复氧模型线粒体膜电位的影响  

Erythropoietin maintains mitochondrial membrane potential and improves energy metabolism of cultured myocardial cell induced by hypoxia-reoxygenation

作　　者：杨雪[1] 秦宇红[2] 朱兵[3] 范利[4] 张丽萍[3] 骆雷鸣[3] 

机构地区：[1]解放军总医院南楼临床部保健科,北京100853 [2]解放军总医院心内科,北京100853 [3]解放军总医院心血管二科,北京100853 [4]解放军总医院南楼临床部,北京100853

出　　处：《老年医学与保健》2007年第2期82-84,共3页Geriatrics & Health Care

基　　金：国家自然科学基金面上项目(编号:30471924)

摘　　要：目的观察EPO对培养的新生大鼠心室肌细胞缺氧-复氧后线粒体膜电位影响,探讨其对缺氧心肌细胞能量代谢的保护作用。方法培养新生大鼠心室肌细胞分成缺氧组和促红细胞生成素(EPO)组。每组根据缺氧-复氧时间分为5个亚组,检测各组细胞线粒体膜电位变化。结果缺氧-复氧后,缺氧组各时间点线粒体膜电位均较未缺氧时下降,但单纯缺氧期下降较缓;复氧后,缺氧组各时间点的膜电位均低于缺氧前;EPO组只在缺氧2h-复氧2h时下降显著;缺氧-复氧后同一时间点两组间比较,EPO组在复氧后的各时间点均高于缺氧组。结论早期应用EPO可明显减轻心室肌细胞缺氧/复氧后线粒体膜电位的下降,促进膜电位的快速恢复,从而改善缺氧心肌细胞的能量代谢。Objective To observe the effect of Erythropoietin （EPO） on mitochondrial membrane potential （MMP） in cultured myocardial cell after hypoxia-reoxygenation, and search for its possible protective effect on cardiolmyocytes＇ energy metabolism. Methods Cultured myocardial cell from neonate rats were divided in hypoxia group A （hypoxla-reoxygenation） and groups B（EPO）. The two groups were further divided into five time points respectively. After hypoxia anti reoxygenation, the cells were stained with fluorescent dyes and MMP was assayed by flow cytometry. Results After hypoxia, MMP of hypoxia group and EPO group all declined. In hypoxia group, MMP of different post-reoxygenation time points were significantly lower than that of pre-hypoxia. In EPO group, only MMP of hypoxia2h/reoxygen2h were lower than pre-hypoxia. MMP of EPO groups were all significamly higher than those of hypoxia groups on each post-reoxygenation time point. Conclusions EPO could preserve and improve recovery of the MMP of hypoxia-reoxygenation injured myocardial cell.

关 键 词：红细胞生成素 缺氧 心肌 线粒体 膜电位 

分 类 号：R541[医药卫生—心血管疾病]