RING3表达沉默细胞系的构建及其对Cyclin A的诱生  

Construction of RING3-Deficient Cell Line and the Inducting Cyclin A by RING3

作　　者：成学[1] 杨复华[1] 

机构地区：[1]武汉大学生命科学学院,湖北武汉430072

出　　处：《武汉大学学报（理学版）》2007年第2期209-212,共4页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30571746)

摘　　要：应用RNA干扰技术,建立了RING3(reallyinteresting gene3)表达沉默的细胞系,研究了RING3内生水平的表达与其可能的靶基因Cyclin A之间的关系.结果表明,在血清饥饿的成纤维细胞NI H/3T3中,用血清刺激后会引起细胞周期蛋白Cyclin A的表达;当用RNAi技术使RING3表达抑制后,Cyclin A的表达受到相应影响,可见Cyclin A的表达受到RING3的调节,其转录激活受到RING3和E2F-1等因子的共同调控,表明RING3与肿瘤发生之间的关系可能与Cyclin A的表达有关.A pSUPER RNAi system was used to construct a RING3-deficient cell line and research on the relationship between RING3 and Cyclin A. Marked expression of Cyclin A was detected when serumstarved NIH/3T3 stimulated with serum. Once RING3 knocked down, it has a same effects on the Cyclin A expression. The Cyclin A transcription is controlled by both RING3 and E2F-1. The deduction that RING3 relates to tumorigenesis can be partly explained by the expression of Cyclin A.

关 键 词：RING3 ( REALLY INTERESTING gene3) 细胞周期因子A 转录激活 细胞周期 

分 类 号：Q291[生物学—细胞生物学]