鹰嘴豆芽素A对小鼠T淋巴细胞体外活化增殖和细胞周期的影响  

Effect of biochanin A on activation,proliferation and cell-cycle of mouse T lymphocytes in vitro

作　　者：林长乐[1] 曾耀英[1] 曾祥凤[1] 赵令斋[1] 黄秀艳[1] 叶雪仪[1] 

机构地区：[1]暨南大学组织移植与免疫实验中心,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2007年第2期115-119,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金重点项目(30230350)

摘　　要：目的:研究黄酮类化合物鹰嘴豆芽素A(biochanin A,bioA)对小鼠T淋巴细胞的活化增殖和周期的影响。方法:运用双色荧光抗体染色技术结合流式细胞术分析bioA对小鼠T淋巴细胞在多克隆刺激剂(ConA)刺激下早期活化抗原CD69表达的影响;运用羧基荧光素乙酰乙酸(carboxyfluorescein diacetate,succinimidyl ester,CFDA-SE)标记技术结合流式细胞术,分析T淋巴细胞增殖相关指数;用碘化丙锭(propidium iodide,PI)染色分析T淋巴细胞细胞周期变化。结果:终浓度为25、50μmol/L的bioA对ConA刺激下的T淋巴细胞早期活化抗原CD69表达具有明显的抑制作用(P<0.05),50μmol/L抑制达到峰值;CFDA-SE染色法显示,25、50μmol/L bioA对ConA诱导的T淋巴细胞增殖作用具有显著的抑制作用(P<0.05);终浓度为5、25、50μmol/L的bioA能够使ConA刺激的T淋巴细胞滞留于G0/G1期,减少处于S期和G2/M期细胞数。结论:BioA对小鼠T淋巴细胞的活化增殖和周期具有明显的抑制作用。Aim.To study the effect of bioehanin A on activation, proliferation and cell-cycle of mouse T lymphocytes. Methods： Effect of biochanin A on activation, proliferation and cell-cycle of mouse T lymphocytes were detected by double-fluorescent,CFDA-SE and PI staining with flow cytometry, respec- tively. Results： Biochanin A can inhibit activation and proliferation of mouse T lymphocytes at the concentration of 25,50μmoL/L, and biochanin A can detain the T lymphocytes in G0/G1 phase ,reduce the cell population of S and G2/M phase. Conclusion： Biochanin A can inhibit the activation, proliferation and cell-cycle of mouse T lymphocytes.

关 键 词：鹰嘴豆芽素A T淋巴细胞 早期活化 细胞增殖 细胞周期 

分 类 号：R392.12[医药卫生—免疫学]