球形棕囊藻溶血毒素对兔红细胞作用的AFM观察  

AFM observation of hemolytic extracts from Phaeocystis globosa on rabbit erythrocytes

作　　者：刘洁生[1] 彭喜春[2] 杨维东[1] 

机构地区：[1]暨南大学生命科学技术学院生物工程系,广东广州510632 [2]暨南大学理工学院食品科学与工程系,广东广州510632

出　　处：《热带海洋学报》2007年第2期55-58,共4页Journal of Tropical Oceanography

基　　金：广东省自然科学基金重点项目(021168);国家自然科学基金(30200030);国家重点基础研究发展规划项目(2001CB409709;2001CB409710)

摘　　要：溶血性毒素是有毒藻类分泌较多的一类毒素,具有溶血活性。运用光学显微镜和原子力显微镜(AFM)观察了球形棕囊藻Phaeocystis globosa溶血毒素对兔红细胞的溶血现象。结果显示,经毒素处理后的兔血红细胞边缘凹凸不平,只剩下一小部分残骸。该毒素可能由于其双亲性质与血红细胞膜表面的膜脂连接,产生脂溶效应,使细胞膜破坏。Many harmful algae can produce hemolytic toxin which is toxic to erythrocytes. The morphological changes of rabbit erythrocytes induced by haemolytic extracts from Phaeocystis globosa were observed with optic microscopy and atomic force microscopy （AFM）. In comparison with normal erythrocytes, significant changes occurred in the ultrastructure of the erythrocytes treated with the extracts. A concavoconvex surface appeared on the cell membrane, suggesting that the haemolytic toxin may break the erythrocytes by combining its lipophilic groups with the membrane.

关 键 词：球形棕囊藻Phaeocystis GLOBOSA 溶血毒素 原子力显微镜 

分 类 号：Q272[生物学—细胞生物学]