HPLC法测定盆炎消凝胶中芍药苷的含量  

Determination of Paeoniflorin in Penyanxiao Gel by HPLC

作　　者：辛义周[1] 于维萍[1] 

机构地区：[1]山东中医药大学附属医院,山东济南250011

出　　处：《齐鲁药事》2007年第4期221-222,共2页qilu pharmaceutical affairs

摘　　要：目的建立测定盆炎消凝胶中芍药苷含量的高效液相色谱法。方法选用Cts枉，流动相为甲醇-0．05mol·L^-1磷酸二氢钠溶液（30：70），检测波长230nm。结果芍药苷在26．4～237．6μg·mL^-1范围内线性关系良好，芍药苷的平均回收率为99．27％，RSD为0．62％。结论方法简便，灵敏，准确，样品处理简便易行，可作为盆炎消凝胶的质量控制方法。OBJECTIVE To determine Paeoniflorin in Penyanxiao Gel. METHODS The chromatographic conditions were as follow：Nucleosil C18 column,a mixture of methanol-0. 05 mol · L^-1 NaH2PO4 （30 ： 70） as the mobile phase and the detection wavelength at 230 nm. RESULTS The calibration curves were linear in the range of 26. 4～237. 6μg·mL^-1 for Paeoniflorin. The average recovery of Paeoniflorin were 99.27%. CONCLUSION This method was accurate, simple and convenient,rapid and reproducible,can be used for the quality control of Penyanxiao Gel.

关 键 词：盆炎消凝胶 芍药苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]