丹参超微饮片水溶性有效部位的HPLC指纹图谱研究  

Fingerprint chromatograms of water-soluble constituents of ultramicro decoction piece of Salvia miltiorrhiza Bge. by HPLC

作　　者：王宇红[1] 张水寒[1] 杨永华[1] 蔡光先[1] 

机构地区：[1]湖南省中医药研究院

出　　处：《中成药》2007年第4期480-483,共4页Chinese Traditional Patent Medicine

基　　金：国家"十五"科技攻关项目(2001BA701A43);湖南省卫生厅中医药科研基金课题(24303)

摘　　要：目的:采用高效液相色谱法(HPLC)对丹参超微饮片水溶性有效部位进行指纹图谱研究。方法:采用Hypersil BDS-C18柱(4.6mm×250mm,5μm),乙腈-0.014(体积分数)磷酸溶液梯度程序洗脱,流速1.0mL/min,柱温25℃,检测波长280nm。以27个共有峰为评价指标,使用“中药色谱指纹图谱相似度评价系统2004A版”软件计算处理。结果:建立的指纹图谱测定系统,对丹参水溶性成分分离良好。将8个产地的10批药材的指纹图谱进行相似度评价,相似系数均在0.9以上。结论:该法操作简便,精密度、稳定性和重复性良好,为丹参超微饮片水溶性有效部位的质量控制提供了方法。AIM： To establish the fingerprint chromatograms for the quality evaluation of Salvia miltiorrhiza Bge. by HPLC. METHODS ： The analysis was performed on a Hypersil BDS-C18 column （5 μm, 4.6 mm × 250 mm） with acetonitrile-water （containing 0. 014% （v/v） phosphat acid） as mobile phase at a flow rate of 1.0 mL/ min, and at a column temperature of 25 ℃. The detection wave-length was at 280 nm. The mutual fingerprint of the extract of Salvia miltiorrhiza Bge was established by using the similarity calculation software of Chinese herbal fingerprint. RESULTS： The HPLC fingerprints of the water-soluble extract of reference S. miltiorrhiza were obtained with very good resolution under the established chromatographic system. The quality of ten batches of S. miltiorrhiza samples from 8 habitats were assessed by comparing their chromatographic fingerprints with the reference fingerprints obtained at the same time, and the similarity showed no difference. CONCLUSION： The method is reliable and can be helpful in effectively controlling the quality of Salvia miltiorrhiza Bge.

关 键 词：高效液相色谱 水溶性成分 指纹图谱 聚类分析 丹参 

分 类 号：R284.1[医药卫生—中药学]