复方五仁醇胶囊含药血清中木脂素类成分的UPLC-MS/MS分析  

Analysis of lignans in the serum of rats after oral administration of Compound Wurenchun Capsules by UPLC-MS/MS

作　　者：窦志华[1] 丁安伟[1] 居文政[2] 罗琳[3] 

机构地区：[1]南京中医药大学药学院,江苏南京210029 [2]南京中医药大学附属医院临床药理科,江苏南京210029 [3]南通大学医学院,江苏南通226006

出　　处：《中成药》2007年第4期550-555,共6页Chinese Traditional Patent Medicine

基　　金：江苏省中医药局中医药科学技术研究专项课题(编号H05173);江苏省高校研究生科技创新计划项目(编号140);江苏省南通市社会发展科技计划项目(编号S40006)

摘　　要：目的:分析复方五仁醇胶囊大鼠灌胃后消化吸收进入血液的木脂素类成分,以便与该制剂血清药理学研究相结合,阐明其药效物质基础。方法:采用UPLC-MS/MS方法,比较复方五仁醇胶囊含药血清、体外制剂、空白血清及对照品提取离子流色谱图(EIC),并通过质谱图相关离子峰分析,确认进入血液的木脂素类成分。结果:发现制剂中存在的五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素5个木脂素类成分进入血液。结论:这5个木脂素类成分很可能是复方五仁醇胶囊体内直接作用物质的重要组成部分,结合血清药理学作进一步研究有助于阐明该制剂药效物质基础。AIM： To illuminate the therapeutic basis of Compound Wurenchun Capsules in combination with serum pharmacology, the lignans of this preparation migrating to blood of rats after oral administration having been accomplished. METHODS： By UPLC-MS/MS method, lignans migrating to blood were affirmed by comparing the extracted ion chromatography （EIC） of the serum containing drug with the ones of Compound Wurenchun Capsules, the control serum and control articles, correlated ion peak in mass-spectrogram were analyzed at the same time. RESULTS： Five lignans migrating to blood have been found, they are schisandrin, schisandrol B, schisantherin, deoxyschizandrin and schisandrin B. CONCLUSION ： Five lignans above mentioned are likely the effective substances of Compound Wurenchun Capsules in human body. More study by means of combining with serum pharmacology will illuminate the therapeutic basis of this preparation.

关 键 词：复方五仁醇胶囊 含药血清 木脂素类成分 超高效液相色谱-串联质谱 

分 类 号：R944.27[医药卫生—药剂学]