孕妇HBV感染模式与宫内感染关系的临床研究  

Relationship of the Modes of Hepatitis B Virus Marker in Pregnant Women with Intrauterine Infection

作　　者：王健[1] 孙琳[2] 侯稳[1] 

机构地区：[1]安徽理工大学医学院病原生物学与免疫学教研室,淮南232001 [2]安徽理工大学医学院护理系教研室

出　　处：《中国计划生育学杂志》2007年第4期223-226,共4页Chinese Journal of Family Planning

基　　金：安徽省教育厅自然科学研究项目(编号:2006KJ147C)

摘　　要：目的了解孕妇乙型肝炎病毒(HBV)感染状况、感染模式及其与新生儿宫内感染的关系。方法ELISA法检测孕妇及部分新生儿血清乙型肝炎血清标志物(HBVM),PCR法检测血清及外周血单核细胞(PBMC)内HBV-DNA。结果孕妇HBV感染率为8.63%(264/3060),HBsAg阳性率为8.19%(251/3060),孕妇HBV感染模式有12种,以“小三阳”模式较多见,占孕妇感染总数的35.98%(95/264)。“大三阳”、“小三阳”孕妇HBV-DNA检出率分别为97.73%(43/44)和26.31%(25/95),差异有显著性意义(P<0.05)。HBeAg(+)、血清HBV-DNA(+)及PBMC中HBV-DNA(+)孕妇宫内感染率分别为46.15%(6/13)、66.67%(8/12)、70.00%(7/10)。结论孕妇中存在一定数量和程度的HBV感染;感染模式以“小三阳”多见,“大三阳”孕妇体内HBV复制水平较高,易发生宫内感染。HBV宫内感染与孕妇HBeAg(+)及HBV-DNA(+)有关。Objective ： To explore the relationship of the modes of hepatitis B virus marker （HBVM） in pregnant women with intrauterine infection. Methods： HBVM and HBV - DNA in serum and peripheral blood mononuclear cells （PBMC） of pregnant women and some of their neonates were detected by the methods of ELISA and PCR, respectively. Results： The total of HBV infection and positive of HBsAg in pregnant women were 8.63% （264/3060） and 8. 19% （251/3060）. Twelve modes had been confirmed and it was more common in the mode of positive of HBsAg, anti - HBe, anti - HBc which was possessed of 35.98% （95/264）. The positive rates of HBV- DNA in HBsAg（ ＋ ）, HBeAg （ ＋ ）, anti -HBc （ ＋ ） mode and HBsAg（ ＋ ）, anti -HBe（ ＋ ）, anti - HBc （ ＋ ） mode were 97.73% （43/44） and 26.31% （25/95）, which showed significantly different （P 〈 0. 05 ）. The HBV intrauterine infection rates were 46. 15% （6/ 13）, 66.67% （8/12） and 70.00% （7/10） in pregnant women with positive of HBeAg, HBV - DNA in serum and HBV -DNA in PBMC. Conclusion： It shows that infection of HBV is presented in some pregnant women and the mode of HBsAg（ ＋ ）, anti - HBe（ ＋ ）, anti - HBc （ ＋ ） is more common. High duplication level of HBV is expressed in positive mode of HBsAg（ ＋ ） , HBeAg （ ＋ ） , anti - HBc （ ＋ ） and easy to induce the intrauterine infection. The intrauterine infection of HBV can be associated with the level of HBeAg （ ＋ ） and HBV- DNA（ ＋ ） in pregnant women.

关 键 词：乙型肝炎病毒 孕妇 宫内感染 

分 类 号：R714.25[医药卫生—妇产科学]