八肽胆囊收缩素抑制脂多糖诱导急性肺损伤小鼠的炎症反应  

Cholecystokinin octapeptide inhibited inflammatory response on mice with acute lung injury induced by lipopolysaccharide

作　　者：倪志宇[1] 丛军 李淑瑾[1] 丛斌[1] 闫玉仙[1] 徐锦荣[1] 高峰[1] 韩冬艳[1] 刘霞[1] 

机构地区：[1]河北医科大学基础医学院,石家庄050017 [2]赵县医院,河北051530

出　　处：《中国急救医学》2007年第4期329-332,共4页Chinese Journal of Critical Care Medicine

基　　金：国家自然科学基金(No.30270529);河北省教育厅科学研究计划项目(No.2006130);河北省博士基金(No.06547008D)

摘　　要：目的探讨八肽胆囊收缩素(cholecystokinin octapeptide,CCK-8)对脂多糖(lipopo- lysaccharide,LPS)诱导急性肺损伤(acute lung injury,ALI)小鼠炎症反应的影响。方法复制LPS致ALI小鼠模型。实验动物随机分为生理盐水对照组、LPS组(腹腔注射LPS)、LPS+CCK-8组(注射LPS前30 min腹腔注射CCK-8)及CCK-8组(单独注射CCK-8)。用酶联免疫吸附法(ELISA)及逆转录-聚合酶链反应(PT-PCR)检测不同时间点各组小鼠血清、肺组织中白细胞介素-1β(IL-1β)、IL-6的含量及mRNA表达情况。观察各组肺组织光镜病理改变。结果腹腔注射LPS可成功复制ALI小鼠模型,肺组织病理显示,在LPS组12h时可见肺间质充血、水肿,大量炎性症细胞浸润,腹腔预注射CCK-8可明显改善肺组织病理变化;腹腔注射LPS可使小鼠血清及肺组织中IL-1β、IL-6表达增加,分别于注射后2h及4h达到高峰,预先注入CCK-8可显著抑制IL-1β、IL-6的表达。结论CCK-8可能通过抑制ALI小鼠IL-1β、IL-6的表达参与抗炎反应过程,从而减轻LPS诱导的ALI小鼠肺组织炎症反应。Objective To investigate the effect of Cholecystokinin octapeptide （ CCK - 8 ） on mice with acute lung injury （ALI） induced by lipopolysaccharide （LPS）. Methods ALI murine model were duplicated by injection of LPS intraperitoneally. Mice were randomly divided into saline control group, LPS group （ injection of LPS intraperitoneally ）, LPS ＋ CCK - 8 group （ injection of CCK - 8 30 min before LPS ） and CCK - 8 group （ injection of CCK - 8 ）. The expressions of interleukin - 1β （ IL - 1β） and IL - 6 in serum and lung tissues were detected by ELISA and RT - PCR. Lung pathological changes were also observed in each group. Results ALI murine model were successfully duplicated by injection of LPS intraperitoneally. Histologically, massive alveolar interstitial tissue edema, hemorrhage, and inflammatory cell infiltration were observed at 12 h of LPS group, and they could be alleviated in LPS ＋ CCK - 8 group. The expressions of IL - 1β, IL - 6 increased markedly in serum and lung of LPS - attacked mice, and reached peak at the time of 2, 4 h after injection of LPS respectively. Pre - treatment of CCK - 8 decreased both IL - 1β and IL - 6 expressions. Conclusions CCK - 8 involved in the anti - inflammatory response by inhibiting the expressions of IL - 1β, IL - 6 in LPS - attacked mice, which Could alleviate the inflammatory response in lung tissue on mice with ALI.

关 键 词：胆囊收缩素 脂多糖类 急性肺损伤 白细胞介素-1Β 白细胞介素-6 

分 类 号：R563.8[医药卫生—呼吸系统]