大鼠鼠胚海马神经细胞原代培养及神经元鉴定  

Primary culture method for hippocampal neurons of embryo of rat and identification of neurons

作　　者：杨明峰[1] 岳伟[2] 张伟[3] 张颜波[1] 孙保亮[1] 牛敬忠[1] 

机构地区：[1]泰山医学院生命科学研究中心,山东泰安271000 [2]泰山医学院附属医院,山东泰安271000 [3]菏泽医学高等专科学校,山东菏泽274030

出　　处：《泰山医学院学报》2006年第7期614-616,共3页Journal of Taishan Medical College

摘　　要：目的建立大鼠鼠胚海马神经元原代培养的方法。方法取孕龄17-18天的Wistar大鼠腹中胎鼠,血清培养法进行海马神经元的原代培养,然后用倒置显微镜进行形态学观察和神经元特异性烯醇化酶免疫组织化学方法进行神经元的鉴定。结果培养8天后的海马神经元经形态学和组织化学方法观察证实神经细胞发育成熟。结论大鼠鼠胚海马神经元原代培养是一种新型的培养方法,具有明显的优势。Objective ： To establish primary culture method for hippoeampal neurons of embryo of rat. Methods ： Embryo of rat which was pregnant for 17 - 18 days was used in experiment. Hippocampal neurons were cultured with Serum combination. Cultured neurons were observed by inverted microscope and identifieated by the method of NSE. Results： Hippocampal neutons were founded to be maturated by the methods of morphology and try. Conclusions ： Primary culture method for hippocampal neurons of embryo of rat is a new method of culture , which is proved to outweigh other methods.

关 键 词：大鼠 海马 神经元 细胞培养 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]