一株融合菌的构建及其调剖特征研究  

Construction of a fusion bacterial strain and the profile control characteristics of it

作　　者：吴庆红[1] 张忠智[2] 俞理[1] 黄立信[1] 杨鹏[1] 

机构地区：[1]石油勘探开发研究院廊坊分院,河北廊坊065007 [2]中国石油大学(北京)化工学院,北京102249

出　　处：《西安石油大学学报（自然科学版）》2007年第2期41-44,共4页Journal of Xi’an Shiyou University（Natural Science Edition）

基　　金：中石油股份公司"十一五"重点科技项目微生物采油技术应用基础研究(06-02C-01-04)

摘　　要：利用原生质体融合的方法构建了微生物采油菌株,并通过其耐温、产糖量等指标筛选了一株优秀的融合子RH18.适应性实验表明,RH18在40℃下能代谢不溶于水的生物多糖,在有杂菌的地层水中也能生长代谢;岩心驱替实验表明,RH18在多孔介质中流动会优先进入大孔道,发酵产糖后能较好地封堵大孔道、调整吸水剖面和提高非均质油层的采收率;从岩心剖面电镜照片上观察到了生物多糖的形状及其分布,分析了融合菌调剖堵水的微观机理.Some kinds of oil recovery bacterial strains are constructed by protoplast fusion.Through investigating their temperature resistance,polysaccharide generating quantity etc.indexes,an excellent strain called as RH18 is selected.The experimental results show that RH18 can adapt to oil formation and metabolize well the microbial polysaccharide at 40 ℃.Core displacement experiments show that after RH18 generating polysaccharide,it can plug big channels in reservoires,control water injection profile and enhance the recovery factor of heterogeneous reservoirs.The shape and distribution of the polysaccharide are observed on scanning electron microscope.The profile control and water plugging mechanism of the fusion bacteria is analyzed.

关 键 词：微生物采油 融合菌 生物多糖 微生物调剖 物理模拟 

分 类 号：TE357[石油与天然气工程—油气田开发工程]