檀香对丹参药代动力学的影响研究  

Influence of Santalum album on the pharmacokinetics of Radix Salvia miltiorrhizae

作　　者：唐一梅[1] 李仲谨[1] 王世祥[2] 刘爱芳[2] 郑建斌[2] 郑晓晖[2] 

机构地区：[1]陕西科技大学化学与化工学院,咸阳712081 [2]西安交通大学-西北大学"中草药现代化研究及工程中心",西安710069

出　　处：《中国药科大学学报》2007年第2期149-152,共4页Journal of China Pharmaceutical University

基　　金：陕西省中医管理局基金资助项目(No.2005103);陕西省重大科技计划攻关基金资助项目(No.2006kz10-G5)~~

摘　　要：目的:研究“丹参饮”中使药(檀香)对君药(丹参)药代动力学参数的影响。方法:建立家兔含药血浆中丹参素含量的高效液相色谱-电喷雾-离子阱质谱(HPLC-ESI-MS/MS)分析方法。血浆用10%高氯酸沉淀蛋白,上清液用乙酸乙酯萃取获得样品。以对羟基苯甲酸为内标,采用离子阱质谱的多级反应模式(MRM)测定丹参素的血药浓度,DAS数据处理软件对数据进行统计学处理。结果:丹参素在0.2-40.0μg/mL范围内线性关系良好,方法检测限为1.0 ng,重复性实验RSD(%)为1.34(n=6),高、中、低3个浓度的平均回收率(%)分别为(80.69±3.93),(95.5±2.81),(74.21±3.58)。配伍给药前后,丹参中丹参素体内代谢动力学模型均为二室开放模型,但配伍给药后AUC0-∞较丹参单独给药明显增大。结论:“丹参饮”中使药(檀香)可促进君药(丹参)吸收入血,该研究可为“君-使”对药体内作用机制的研究提供新思路。Aim： To study the influence of Santalum album （Shi herb） on the pharmacokinetics of Radix Salvia miltiorrhizae （Jun herb ） in the rabbits receiving Danshenyin. Methods： A valid assay for the determination of Danshensu was established by HPLC-ESI-MS/MS. The plasma was pretreated with 10% perchlorie acid and the supernatant was extracted by ethyl acetate. The p-hydroxybenzoic acid was used as an internal standard to detect the blood-drug concentrations by HPLC-ESI-MS/MS. The collected data were processed by computer program DAS to study the effects of Santalum album on the pharmaeokineties of Radix Salvia miltiorrhizae. Results： The linear range of Danshensu in the assay was 0.2 - 40.0 μg/mL. The limit of detection （LOD） was 1.0 ng. The RSD as reproducibility in experiment was 1.34% （ n = 6）. The average recovery at lower, middle, and upper spiked concentrations was （74.21 ± 3.58） %, （95.5±2.81 ） % and （80.69±3.93 % ）, respectively. Moreover, prior to and post-oral dosing of compatibility, the pharmacokinetics of Dansbensu was found to be fitted to open two-compartment model. And co-administration resulted in obvious increase in AUCo-∞. Conclusion： Shi herb could promote the absorption of Jun herb in Danshenyin. The results could provide clues for understanding of phamaaeologieal mechanism of Jun-Shi pair herbs.

关 键 词：丹参 檀香 药代动力学 “君-使”对药 

分 类 号：R285[医药卫生—中药学]