一个蒙古族人群HLA-A位点DNA多态性分析  

HLA-A Genetic Polymorphism in a Chinese Mongolian Population

作　　者：王帆[1] 田伟[1] 李立新[1] 蔡金洪[1] 色音图 佟豪[3] 陈然[4] 罗奇志[1] 孙科柱[5] 曾庆仁[5] 郭实士[1] 

机构地区：[1]中南大学基础医学院免疫学教研室 [2]内蒙古包钢预防保健中心 [3]内蒙古医学院第三附属医院包钢医院内分泌科 [4]内蒙古医学院第三附属医院包钢医院影像中心 [5]中南大学基础医学院细胞与分子生物学实验中心

出　　处：《实用预防医学》2007年第2期292-293,共2页Practical Preventive Medicine

基　　金：中南大学留学归国人员科研启动基金资助

摘　　要：目的人类白细胞抗原(Human Leucocyte Antigen,HLA)编码基因具有高度遗传多态性,有关中国人群HLA分布,已有的文献报道主要为汉族人群数据。本工作首次采用DNA分型技术,分析蒙古族HLA-A位点多态性。方法采用ARMS/PCR技术对83例居住在内蒙古地区的蒙古族健康个体做HLA-A DNA分型。结果该人群存在HLA-A*01至A*33等11种等位基因(组),其中,以HLA-A*02频率最高(0.2886),HLA-A*11、A*24次之;该人群中,HLA-A19宽特异性(Broad Specificity)含有HLA-A*29、A*30、A*31、A*32、A*33等5组编码基因,频率之和为0.2368;此外,在蒙古族,HLA-A*01较为常见(基因频率为0.08823)。结论本研究在DNA水平提供了蒙古族HLA-A位点频率分布数据。Objective The genes encoding human leucocyte antigen are highly polymorphic, however, previous studies regarding the HLA distribution in Chinese populations have mainly focused on Chinese Han populations residing at various geographic regions. Our study was designed to explore the HLA- A genetic polymorphism of Chinese Mongolian population at DNA level. Methods Eighty - three unrelated healthy individuals of Mongol, recruited from Inner Mongolia, were typed for HLA- A locus by using amplification refractory mutation system - polymerase chain reaction（ARMS/PCR） technique. Results Eleven HLA- A alleles（groups） were found, the most frequent alleles were HLA - A ＊ 02, A ＊ 11, and A ＊ 24, with gene frequencies of 0. 2886, 0. 1713, and 0. 1569 respectively. In this sample panel, HLA- A19 broad specificity was composed of HLA- A＊ 29, A ＊ 30, A ＊ 31, A ＊ 32, and A＊ 33 splits, which were summed to a frequency of 0. 2368. In addition, HLA - A ＊ 01 was a relatively common allele with gene frequency of 0. 08823. Conclusion Our study provides the data of HLA- A genetic polymorphism of a Chinese Mongolian population at DNA level.

关 键 词：白细胞抗原 HLA—A 蒙古族 PCR/SSP 

分 类 号：Q987[生物学—遗传学] R394[生物学—人类学]