基层医院血浆凝固酶阴性葡萄球菌感染现状和耐药性分析  

Analysis of Nosocomial Infection and Antibiotic Resistance of Coagulase Negative Staphylococcus in a Primary Hospital

作　　者：李建芳 

机构地区：[1]广东省中山市阜沙医院检验科,广东中山528434

出　　处：《实用预防医学》2007年第2期361-362,共2页Practical Preventive Medicine

摘　　要：目的研究血浆凝固酶阴性葡萄球菌(CNS)的感染现状和耐药情况,为临床医生合理使用抗生素,提供试验依据。方法按照《全国临床检验操作规程》进行细菌分离培养,采用法国生物梅里埃公司生产的API-staph细菌鉴定系统进行菌种鉴定,药敏试验采用纸片扩散法(K-B法)。结果共检出CNS6种135株,以表皮葡萄球菌和木糖葡萄球菌为主要菌种,耐苯唑西林株检出率为73.3%(99/135)。耐药率监测表明,耐苯唑西林葡萄球菌(MRCNS)株对15种抗生素耐药率远高于苯唑西林敏感葡萄球菌(MSCNS)株,各种CNS对万古霉素和替考拉宁均敏感。结论病原学监测是检测和控制CNS感染的有效方法,应重视MRCNS的检测和报告,根据细菌的药敏结果合理选择抗菌药物。Objective The clinical distribution and antibiotic resistance of coagative negative staphylococcus（CNS） were reviewed to provide experimental basis for correct and efficient clinical antibiotic therapy. Methods CNS was isolated and cultured according to ＂The Regulation of General Clinical Laboratory Operation＂ and tested by API staph system. Disc diffusion test（K - B method） was used to study the antimicrobial resistance. Results One hundred and thirty- five strains of CNS were isolated. They were classified into 6 species of CNS with chemical technique. Staphylococcus epidermidis and staphylococcus xylosus accounted for the majority. Methocillin resistant coagulase negative staphylococcus（MRCNS） accounted for 73.3% （99/135）. Vancomycin and teicoplanin were all effective to CNS. The drug sensitivity results showed that MRC- NS was highly resistant to fifteen antibiotics than methocillin - sensitive coagulase negative staphylococcus（MSCNS）. Conclusions Etiologic monitoring is an effective way to detect and control the infection of CNS. Clinical microbiology laboratory should pay great attention to MRCNS detection. Antimicrobial therapy should be conducted according to susceptibility test.

关 键 词：血浆凝固酶 葡萄球菌 抗生素 耐药性 

分 类 号：R378.11[医药卫生—病原生物学] R978.1[医药卫生—基础医学]