固相萃取-高效液相色谱法测定人血清中莫西沙星浓度  

Determination of moxifloxacin in serum by solid phase extraction HPLC

作　　者：张吟[1] 陈一农[1] 陈文发[1] 

机构地区：[1]福建医科大学附属第二医院,福建泉州362000

出　　处：《中国医院药学杂志》2007年第4期481-483,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的：建立高效液相色谱法检测人血清中莫西沙星浓度。方法：血清经固相萃取后，在Waters Symmetry shield RP18色谱柱上，以乙腈-10mmol·L^-1磷酸二氢钾溶液（19：81）为流动相，流速1．0mL·min^-1，296nm波长检测。结果：莫西沙星的保留时间：08．82min，最低定量限为0．05mg·L^-1，最低检测限0．015mg·L^-1，线性范围0．05～5．00mg·L^-1，萃取回收率91．05％～98．40％，方法回收率96．05％～101．05％，日内RSD2．88％～4．44％，日间RSD5．22％～9．53％。结论：该法准确可靠，可用于莫西沙星的临床药动学研究及临床特殊人群的血药浓度测定。OBJECTIVE To establish a new solid phase extraction-HPLC method for determination of moxifloxacin in hunan serum. METHODS Serum was extracted by a solid phase extraction（SPE） cartridge. The separation was performed on a Waters Symmetry shield RP18 column with mobile phase of acetonitrile-10 mmol·L^-1 potassium dihydrogenphosphate buffer solution （19：81）. The flow rate was 1.0 mL·min^-1 and the detection was set at 296 nm. RESULTS The parameters of moxifloxacin were as follows： tR was 8. 82 min; limit of quantitation （LOQ） was 0. 05 mg·L^-1 in serum; limit of detection（I.OD）was 0. 015 mg·L^-1, the linear relation was in the range of 0. 05 - 5. 00 mg·L^-1 ;the recovery of extraction was 91.05 % -98. 40%; the recovery of methodology was 96. 05% - 101.05 %;the RSD for within-day and between-day were 2. 88% -4. 44% and 5. 22 % - 9. 53 %, respectively. CONCLUSION The method could be used for pharmacokinetic studies and the determination of moxifloxacin in serum of patients.

关 键 词：莫西沙星 固相萃取 高效液相色谱法 血药浓度 

分 类 号：R927.2[医药卫生—药学]