深圳地区女性感染人乳头瘤病毒基因类型分析  

Analysis of the Genotyping Human Papillomavirus from Shenzhen Women

作　　者：何桂蓉 武学成 

机构地区：[1]暨南大学医学院第二附属医院深圳市人民医院检验科,广东深圳518020

出　　处：《现代检验医学杂志》2007年第2期19-21,共3页Journal of Modern Laboratory Medicine

摘　　要：目的 了解深圳地区各年龄段与不同生殖道疾病女性感染人乳头瘤病毒（human papillomavirus，HPV）基因类型分布状况，为深圳地区HPV分子流行病学研究提供依据。方法 利用反向杂交技术分别对2005年1月～2005年12月来医院就诊的801例可疑患者进行23种HPV基因亚型检测，其中包括18种高危亚型（HPV16，18，31，33，35，39，45，51，52，53，56，58，59，66，68，73，83，MM4）及5种低危亚型（HPV6，11，42，43，44）。结果HPV阳性检出率为17．5％（140／801），其中高、低危型检出率分别为16．9％（135／801）与2．6％（21／801），高危型中主要为HPV16，58感染，低危型中主要为HPV11感染。不同生殖道疾病（宫颈炎性病变、尖锐湿疣、宫颈上皮内瘤变（≥CIN））与正常对照组比较差异有显著性意义（P〈0．05）。20-30y，31-40y，40～50y和〉50y各年龄组中HPV阳性的比率为32％，38．5％，22．4％和7．1％。结论 HPV11，16，58是深圳地区HPV感染的主要型别，20-50y为感染的高峰人群，对HPV阳性者进行定期的跟踪，是防治尖锐湿疣与宫颈癌的重点。Objective Learning the distribution of HPV types in different age groups and various cervical lesions provided datum for HPV molecule epidemiology of Shenzhen area. Methods 801 patients from January to December 2005 were analysed. 23 types of HPV DNA including 18 high-risk HPV types （HPV16/18/31/33/35/39/45/51/52/53/56/58/59/66/ 68/73/83/MM4） and 5 low-risk HPV types （HPV6/11/42/43/44） were detected in cervical samples by using reverse dot blot hybridization technology. Results HPV genotyping by reverse dot blot hybridization revealed that 140 positive samples were found in 801（17.5%） women cervical swabs and the detected rates of low-risk HPV and high-risk HPV were 16.9 % and 2.6 % ,respectively. HPV types 16 and 58 were the most frequent type in all specimens ,and HPV types 59,33, 11 and 18 followed by. The cervical inflammatory lesions ,condyloma acuminatum,cervical intraepithelial neoplasia（≥CIN） were significant difference compared with the normal group（P〈0. 05）. The positive rates of HPV at different age groups, aged 20-29,30-39,40-49,-50 years were 32% ,38.5% ,22.4% and 7.1- ,respectively（P〈0. 05）. Conclusion HPV types 16 and 58 are the most frequent type and people aged 20-50 years are the most frequent infected patients in Shenzhen area. Tracking regularly HPV infection patients is important in prevention and cure of wart and cervical cancer.

关 键 词：人乳头瘤病毒 分型 病毒DNA 

分 类 号：Q754[生物学—分子生物学] R373[医药卫生—病原生物学]