三氯乙烯职业中毒皮肤损害与HLA-DR、DQ基因相关性研究  

Study on the Correlation between the Skin Lesion Caused by Occupational Poisoning of Trichloroethylene and HLA-DR, DQ Genes

作　　者：庾蕾[1] 庄志雄[1] 黄海雄[1] 俞慕华[1] 叶小明[1] 谢富焕[1] 

机构地区：[1]深圳市疾病预防控制中心食品(毒理)科,广东深圳518020

出　　处：《环境与职业医学》2007年第2期133-135,共3页Journal of Environmental and Occupational Medicine

基　　金：国家重点基础研究发展计划(973计划)(编号:2002CB512904);深圳市科技计划项目(编号:302)

摘　　要：[目的]从HLA-DR、DQ基因角度探讨三氯乙烯职业中毒皮肤损害易感的遗传背景。[方法]采用PCR- SSP(sequence specific primer)法对三氯乙烯职业中毒皮肤损害组(病例组)及对照组进行HLA-DR、DQ基因位点等位基因分型。[结果]HLA-DRB1位点在病例组检出11个等位基因,11个血清型;对照组检出13个等位基因,13个血清型。HLA-DQB1位点在病例组及对照组均检出5个等位基因,7个血清型。获得了各等位基因分布及基因频率资料,其中DRB1*09(DR9)和DQB1*03(DQ9)等位基因频率在病例组与对照组间差异均有显著性(P<0．05);OR值(95％可信区间)分别为DRB1*09(DR9)等位基因为0．174(0．037～0．830),DQB1*03(DQ9)等位基因为0．226(0．046～1．108)。[结论] DRB1*09(DR9)和DQB1*03(DQ9)等位基因可能是三氯乙烯职业中毒皮肤损害的保护基因。[ Objective ] To study the genetic background of susceptibility to skin lesion caused by occupational poisoning of trichloroethylene from the viewpoint of HLA-DR, DQ genes. [ Methods ] The alleles were genotyped at HLA-DR, DQ loci of the skin lesion（ case ） group and control group using PCR-SSP sequence specific primer method. [ Results ] Total 11 alleles and 11 serotypes at HLA-DRB1 locus were identified in case group whereas 13 alleles and 13 serotypes identified in control group. At HLA-DQB1 locus 5 allels and 7 serotypes were identified in both groups. The data of the distribution and frequency of alleles were obtained. Among these alleles, the frequencies of alleles, DRB1＊09（ DR9 ） and DQB1＊03 （ DQ9 ） had significant difference between the case group and control group（P〈0.05 ）. The 0R（95% C/）were 0.174（0.037-0.830） and 0.226（0.046-1.105 ） respectively. [ Conclusion ] TheDRB1＊09 （ DR9 ） and DQB1＊03 （ DQ9 ） alleles may be the protecting genes to skin lesion caused by the occupational poisoning of trichloroethylene.

关 键 词：三氯乙烯 皮肤损害 人类白细胞抗原 基因多态性 

分 类 号：R135.1[医药卫生—劳动卫生] Q786[医药卫生—公共卫生与预防医学]