焦炉工人血浆BPDE-白蛋白加合物与尿中1-羟基芘浓度的关系  

The Relationship between Plasma BPDE-albumin Adduct and Urinary 1-Hydroxypyrene Levels among Coke Oven Workers

作　　者：王红[1] 刘爱林[1] 郑红艳[1] 郭亮[2] 梁华山[2] 杨晓波[1] 白云[1] 陈永文[1] 王静[1] 谭皓[1] 邬堂春[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系和教育部环境与健康重点实验室 [2]太原钢铁有限公司疾病预防控制中心,山西太原030027

出　　处：《环境与职业医学》2007年第2期140-142,共3页Journal of Environmental and Occupational Medicine

基　　金：国家"973"计划基金资助项目(编号:2002CB512905);国家自然科学基金资助项目(编号:30371204)

摘　　要：[目的]探讨血浆中苯并(a)芘二氢二醇环氧化物(BPDE)-白蛋白加合物作为焦炉工人多环芳烃长期暴露的生物标志物的可行性及其与尿中1-羟基芘(1-OHP)浓度之间的关系。[方法]采用反相高效液相色谱方法,对焦化厂37名焦炉作业工人(接触组)和47名非焦炉作业人员(对照组)血浆中BPDE-白蛋白加合物和尿中1-羟基芘浓度分别进行测定。[结果]接触组血浆中BPDE-白蛋白加合物和尿中1-羟基芘浓度的中位数分别为42．10fmol/mg白蛋白和5．46μmol/mol肌酐,均显著高于对照组(14．16μmol/mg白蛋白,2．96μmol/mol肌酐,P<0．01)。校正了个体的年龄、吸烟和饮酒状况后,接触组BPDE-白蛋白加合物浓度增高的危险度是对照组的1．79倍(P<0．05)。接触组1-羟基芘浓度增高的危险度是对照组的2．45倍(P<0．05)。并且在所有研究对象中,BPDE-白蛋白加合物与尿中1-羟基芘浓度存在显著的正相关关系(rs=0．349,P<0．01)。[结论]血浆中BPDE-白蛋白加合物与尿中1-羟基芘水平显著相关,BPDE-白蛋白加合物可作为反映多环芳烃较长时期暴露的接触生物标志物。[Objective] To investigate the application of BPDE-albumin adduct as the monitoring biomarkers for cok oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) and the relationship between this adduct and the urinar 1-hydroxypyrene (1-OHP) levels in the same workers. [Methods] 37 coke oven workers from a coke plant and 47 control without occupational PAHs exposure were recruited in this study. The levels of plasma BPDE-albumin adduct and urinar 1-hydroxypyrene were analyzed using high performance liquid chromatography. [Results] The median concentration of BPDE Alb adducts in exposed group(42.10 fmol/mg albumin)was significantly higher than that in control group(14.16 fmol/mg albumin P<0.01). The median urinary 1-OHP in exposed and control groups were 5.46 and 2.96μmol/mol creatinine respectively(P<0.01) The multiple logistic regression analysis showed that coke oven workers were at the higher risk of having BPDE-albumin adduc levels(OR=1.79, P<0. 05)and urinary 1-OHP levels(OR=2.45, P<0. 05). A positive correlation between the levels of BPDE albumin adduct and urinary 1-hydroxypyrene in all subjects was observed (rs=0.349, P<0.01). [Conclusion] Plasma BPDE albumin adduct levels were significantly associated with urinary 1-hydroxypyrene levels. Plasma BPDE-albumin adduct is an usefu biomarker for monitoring longer-term exposure to PAHs.

关 键 词：多环芳烃 BPDE.白蛋白加合物 1-羟基芘 

分 类 号：R114[医药卫生—卫生毒理学]