MRI评价自体骨髓间充质干细胞移植治疗AMI效果的实验研究  

Assessment of the effects of autologus bone MSCs transplantation in acute myocardial infarction by using MR imaging

作　　者：刘孝钧[1] 马根山[1] 祁春梅[1] 胡耀鹏[1] 李璇[1] 苏亚民[1] 

机构地区：[1]东南大学临床医学院,江苏南京210009

出　　处：《现代医学》2007年第2期136-138,共3页Modern Medical Journal

基　　金：国家自然科学基金资助项目(30570743)

摘　　要：目的探讨移植自体骨髓间充质干细胞对急性心肌梗死的治疗效果。方法选取10只2月龄小型家猪抽取骨髓,分离培养自体骨髓间充质干细胞,经外周动脉介入法制备小型猪的心肌梗死模型。造模成功后7 d经冠状动脉注入自体骨髓间充质干细胞(实验组)以及生理盐水(对照组),分别在心肌梗死后7 d和移植后1个月行核磁共振(MRI)检查,评价心功能的变化。结果移植后1个月实验组左心室射血分数(0.50±0.06)明显高于对照组(0.39±0.09)(P<0.05)。结论自体骨髓间充质干细胞移植治疗急性心肌梗死可以明显改善梗死区心肌收缩功能,MRI可以显示心肌梗死的透壁程度及梗死区的范围,可以通过测量舒张末期容积与收缩末期容积来计算左室的射血分数,反映左心功能变化。Objective To explore the effects of autologus bone marrow mesenchymal stem cells （MSCs） transplantation improving the cardiac function after acute myocardial infarction（AMI） in pig. Methods MSCs of ten pigs were collected and cultured in DMEM medium. AMI model was made by blocking blood stream of left anterior descending branch. After 7 days, culture-expanded autologus bone MSCs （ experiment group ） or normal saline（ control group）were transplanted into each host heart＇s AMI area through intracoronary way. MRI was performed to observe the left ventricular function at 7 days after AMI and 1 month after transplantion. Results After 1 month,left ventricular ejection fraction（LVEF） of experiment group（0.50 ± 0. 06） was significantly higher than that in control group （0.39 ±0.09 ） （ P 〈 0.05 ）. Conclusion Autologus bone MSCs transplantation can improve the left ventricular function of pigs after AMI. MRI can detect the scope of AMI area and evaluate left ventricular function by calculating LVEF.

关 键 词：骨髓间充质干细胞 急性心肌梗死 核磁共振成像 猪 

分 类 号：R542.22[医药卫生—心血管疾病] R457.7[医药卫生—内科学]