HPLC法测定珍菊降压片中蒙花苷含量  

Determination of Linarin in Zhengjujiangya Tablet by RP-HPLC

作　　者：陈翠英[1] 袁子民[1] 程岚[1] 陈剑锋 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁成大方圆医药连锁有限公司,辽宁沈阳110001

出　　处：《辽宁中医杂志》2007年第4期501-502,共2页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:建立HPLC法测定珍菊降压片中蒙花苷含量。方法:采用ZORBAX Eclipse XDB-C18色谱柱,以乙腈-水(28∶72)为流动相,检测波长334nm。结果:蒙花苷在0.025～0.50μg线性关系良好,r=0.9994,平均回收率为98.7%(n=5),RSD为2.1%。结论:本法简便、准确、重复性好,可用于珍菊降压片中蒙花苷含量测定。Objective： To establish HPLC for determining the content of linarin in Zhengjujiangya Tablet. Method： A HPLC method was developed. The chromatographic conditions are as follow ZORBAX Eclipse XDB - C18 column and acetonitrile - water （25： 75） as mobile phase, detection wavelength at 334nm. Result ： The linear range of linarin was 0.025 - 0.05μg. The average recovery rate was 98.7% and RSD 2.7%. Conclusion： The method is simple and accurate with good repeatability, and can be used for determinate linarin in Zhengjujiangya Tablet.

关 键 词：珍菊降压片 蒙花苷 HPLC 

分 类 号：R284.1[医药卫生—中药学]