抗纤复方Ⅰ号对酒精性肝病大鼠肝星状细胞活化的影响  

Effet of Kangxian Fufang-Ⅰ on the initiation of hepatic stellate cells in rats with alcoholic liver disease

作　　者：崔巍[1] 刘冬娟[2] 林红[1] 傅宝玉[1] 

机构地区：[1]中国医科大学附属第一医院消化内科,辽宁沈阳110001 [2]中国医科大学第二电镜室,辽宁沈阳110001

出　　处：《中西医结合肝病杂志》2007年第2期102-104,F0003,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:观察抗纤复方Ⅰ号对酒精性肝病大鼠肝星状细胞活化的影响,以探讨中药治疗酒精性肝病的机制。方法:给予Wistar大鼠乙醇8～10g.kg-1.d-1,分3次灌胃,12周制备酒精性肝病大鼠模型,对照组给予等量生理盐水,中药组在给予同酒精组等量乙醇的基础上,给予抗纤复方Ⅰ号水煎剂4.0ml.kg-1.d-1,分2次灌胃,共12周。实验4、8、12周末分批处死动物,HE及VG染色观察肝脏组织学改变,电镜下观察肝星状细胞形态学改变并检测肝内透明质酸(HA)和层黏连蛋白(LN)含量。结果:造模12周时,酒精组大鼠肝脏呈现重度脂肪变性,点片状肝细胞坏死及炎性细胞浸润,纤维条索形成;而中药组大鼠仅呈现轻度脂肪变,无纤维条索形成。电镜下酒精组大鼠肝细胞失去正常结构,星状细胞增多、变形,细胞外可见较多胶原纤维。中药组大鼠肝细胞及星状细胞形态基本正常,基质内亦可见少量胶原纤维。实验8周末酒精组LN浓度开始升高,12周末达到最高,与正常组及中药组比较差异有显著性意义[(37.69±5.01)vs(22.34±3.51)、(28.07±4.11),P<0.05];肝内HA浓度8周末各组间比较无统计学差异,12周末酒精组明显升高,与正常组及中药组比较,差异有显著性意义[(74.85±9.23)vs(41.26±8.34)、(53.62±4.85),P<0.01]。结论:抗纤复方Ⅰ号能降低肝内HA和LN,抑制酒精性肝纤维化形成,这与其抑制肝星状细胞活化有关。Objective： To investigate the effect of Kangxian Fufang Ⅰ （ KX Ⅰ） on hepatic stellate cells in rats with ethanol-induced liver disease. Methods： Wistar rats weighing between 160g and 200g were intragastricly fed with ethanol （8 to 10g · kg^-1 · d^-1 ） or ethanol plus KX Ⅰ （4. 0ml · kg^-1· d^-1 ） for 12 weeks. Control rats received normal sodium instead of ethanol. At the end of 4, 8, 12w, rats were killed and received liver histopathology and ultrastructure test. Hepatic hyaluronic acid （HA） and laminin （LN） were measured by radioactive immunoassay. Results. Up to 12 weeks, marked hepatic fatty changes, inflammation, necrosis and fibrosis were observed in ethanol-fed rats. In contrast, these alternations were attenuated by KX Ⅰ administration although mild fatty changes remained. By electron microscope, liver ultrastructure was observed that hepatic stellate cells （HSC） increased, became myofibroblast-like cells and there was a large of collagen around cells in ethanolfed rats whereas HSC remains normal shape and only a little of collagen was observed in medicine-treated rats. At the end of 12 weeks, hepatic HA and LN reached the highest levels in ethanol-fed rats and were significantly higher than controls and medicine-treated groups （74. 85 ± 9. 23 vs 41.26 ± 8. 34. 53.62 ± 4. 85, P 〈 0. 01 ; 37. 69 ± 5. 01 vs 22. 34 ± 3.51. 28.07 ± 4. 11, P 〈 0. 05）. Conclusion： KX Ⅰ decreased the levels of hepatic HA and LN, attenuated ethanol-induced hepatic fibrosis in rats. The mechanism was contributed to inhibit hepatic stellate cells initiation.

关 键 词：酒精性肝病 大鼠 抗纤复方Ⅰ号/药效学 肝星状细胞 透明质酸 层黏连蛋白 

分 类 号：R285.5[医药卫生—中药学]