植物体内MDA不同提取液与显色液反应条件的比较  

Comparisons on Reaction Conditions of Different Extraction Solvents for Plant Endogenous MDA and Chromogenic Reagent

作　　者：刘少霞[1] 陈贵[1] 秦萍[1] 曹冬煦[1] 马冬伍 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110161 [2]辽宁省沈阳市沈北新区疫病预防控制中心,辽宁沈阳110121

出　　处：《安徽农业科学》2007年第10期2829-2829,2832,共2页Journal of Anhui Agricultural Sciences

摘　　要：用50 mmol/L磷酸缓冲液pH值7.8、去离子水、5%三氯乙酸3种提取液分别提取小苍兰叶片中的MDA,分别与5%三氯乙酸+0.5%TBA1、mol/L HCl+0.5%TBA、30%乙酸+0.5%TBA 3种显色液进行交差显色试验。结果表明:用去离子水提取植物体内的MDA效果较好,用30%乙酸+0.5%TBA与MDA显色,反应产物在特定波长(532 nm)处有明显吸收峰值。Three extraction solvents of 50 mmol/L phosphate butler at pH 7.8, deionized water, 5% TCA were used to extract MDA in leaves of Freesia refracta respectively, The MDA extracted by three extraction solvents were reacted with three chromogenic reagents of 5%TCA ＋ 0.5%TBA, 1 mol/L HCl ＋ 0.5%TBA, 30%HAC ＋ 0.5%TBA, respectively, The results showed that deionized water had better effect of extracting plant endogenous MDA and its reactions products with chromogenic reagent of 30% TCA ＋ 0.5% TBA had a remarkable absorption peak at the wavelength of 532 nm.

关 键 词：MDA 提取液 显色液 植物 

分 类 号：Q946[生物学—植物学]