垂吊矮牵牛的组织培养和快速繁殖  

Tissue Culture and Rapid Propagation of Petunia hybrida

作　　者：张瑞越[1] 季勤[1] 白莹[1] 邢宇俊[1] 

机构地区：[1]淮阴师范学院生物系,江苏淮安223300

出　　处：《安徽农业科学》2007年第10期3003-3004,共2页Journal of Anhui Agricultural Sciences

摘　　要：以垂吊矮牵牛（Petunia hybrida）叶片为外植体，研究了在培养基中添加不同激素成分及不同浓度对丛生芽形成和生根的影响。结果表明，直接诱导丛生芽的最佳培养基为MS＋6-BA2．0mg/L＋NAA0．3mg/L，最适生根培养基为1／2MS＋NAA0．05～0．1mg/L。外植体接种40d左右便可形成完整的再生植株。With the leaf of Petunia hybrida as explant, the effect of medium adding different hormone component with different concentration on cespitose bud and rooting was studied. Results showed that the optimum bud-inducing medium was MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.3 mg/L and the most suitable rooting medium was 1/2MS ＋ NAA 0.05 -0.1 mg/L. The intact regeneration seedlings were formed after the explants cultured for 40 d.

关 键 词：垂吊矮牵牛 外植体 丛生芽 再生体系 

分 类 号：Q943.1[生物学—植物学]