缺血后处理、ATP后处理减轻兔缺血再灌注损伤:与腺苷受体激活有关  

Cardio-protection of ATP-postconditioning and Postconditioning in Rabbits: Associated with the Activation of Adenosine Receptors

作　　者：刘方[1] 廉哲勋[1] 王永彬[2] 葛毅萍[3] 

机构地区：[1]青岛大学医学院附属医院心内科,青岛266003 [2]青岛大学医学院附属医院急诊科,青岛266003 [3]青岛大学医学院,青岛266003

出　　处：《现代生物医学进展》2007年第3期353-355,共3页Progress in Modern Biomedicine

摘　　要：目的:近期实验研究显示,在再灌注的早期给予短暂、重复的缺血再灌(缺血后处理Postconditioning)能够减轻心肌再灌注损伤。本实验旨在探明三磷酸腺苷(ATP)用于缺血后处理是否产生上述保护效应,以及了解腺苷受体在此保护作用机制中的地位。方法:家兔开胸后左前降支均给予40min结扎和180min的再灌注,并随机分为5组:(1)对照组;(2)缺血后处理组;(3)ATP后处理组;(4)缺血后处理+SPT(硫苯茶碱)组;(5)SPT对照组。于实验终点测定心肌梗死面积(TTC染色),血浆CK-MB、SOD、MDA含量。结果:和时照组相比,缺血后处理组与ATP后处理组心梗面积减少(p<0．05),CK-MB也显著降低(p<O．05)。在SPT+后处理组,SPT消除了缺血后处理的上述减少心梗面积作用(p<0．05)。此外,在两个后处理组MDA含量明显降低(p<0．05),SOD含量明显升高(p<0．05),同样地,在SPT+后处理组发现SPT取消了缺血后处理的减轻再灌注损伤作用。结论:ATP后处理具有与机械后处理相同的减轻心肌缺血再灌注损伤作用,而SPT消除缺血后处理保护作用的现象提示,该保护作用的机制可能与腺苷受体的激活有关。Objective： Recent experimental studies indicate that application of short, repetitive ischemia only during the onset of reperfusion, i.e, postconditioning （Post-con）, attenuates myocardial reperfusion injury. This study was designed to examine whether Adenosine Triphosphate （ATP） has the same cardio-protective effects or not when administered at the onset of reperfusion, and investigate the role of adenosine receptors in the protection by Post-con. Methods： Thirty anesthetized open-chest rabbits were subjected to 40 rain ischemia of the left anterior descending artery （LAD） and 180 rain reperfusion, and assigned to the following five groups： （1）Control; （2）Post-con; （3）ATP-Post; （4） Post ＋ SPT; （5）CON＋SPT, Infarct size （TTC staining）, the plasma CK-MB, superoxide dismutase （SOD）, and lipid peroxidation product malondialdehyde （MDA） were measured at the end of ischemia, Results： Compared with Control, myocardial infarct size was limited （p〈0,05）, which was consistent with CK-MB （p〈0,05）; SPT alone had no effect on infarct size（CON＋SPT vs CON, p〉0.05）, However, Post＋SPT abrogated the myocardial infarct size reduction in Post-con （p〈0.05）. Besides, MDA was lower and SOD higher in Post-con and ATP-Post than those in CON （p〈0.05）, respectively, Similarly, Post＋SPT abolished these protective effects in Post-con. Conclusions： Our results suggest that ATP can protect the heart from myocardial ischemia/reperfusion injury when administered at the onset of reperfusion and is as effective as mechanical postconditioning. The ability of SPT to block the cardio-protective effects indicates the mechanism of postconditioning＇s protection may be associated with the activation of adenosine receptors.

关 键 词：缺血再灌注损伤 缺血后处理 腺苷受体 

分 类 号：R5[医药卫生—内科学]