矮牵牛Tidal Wave品种遗传转化受体再生体系的建立  

Establishment of Regeneration System for Genetic Transformation for Common Garden Petunia'Tidal Wave’

作　　者：武术杰[1] 李邱华[2] 

机构地区：[1]长春大学,长春130031 [2]中国农业大学

出　　处：《东北林业大学学报》2007年第4期14-15,27,共3页Journal of Northeast Forestry University

摘　　要：以矮牵牛Tidal Wave品种无菌苗叶片为外植体,在附加不同质量浓度激素的MS基本培养基上诱导培养,通过器官发生途径获得再生植株。在附加3.0mg/LBA、0.3mg/LNAA的培养基上获得了90%以上芽的再生率。再生芽在附加0.02mg/LNAA、1.0mg/LKT、5.0mg/LGA3的培养基上发育良好。在3种选择抗生素的筛选中发现,叶片对潮霉素和庆大霉素较为敏感,这2种抗生素质量浓度为5mg/L时,2周内可将外植体全部杀死;而卡那霉素质量浓度达到15mg/L时,芽分化才被完全抑制。因此可确定卡那霉素为理想的选择抗生素,其选择压质量浓度为15mg/L。2种抑菌抗生素中,头孢霉素对叶片再生影响较大;而250mg/L的羧苄青霉素能有效地抑制农杆菌菌株LBA4404的生长,却对矮牵牛叶片的芽分化影响不大,为适宜的抑菌抗生素。Regeneration plantlets were obtained from young leaf explants of the petunia＇ tidal wave＇ induced on MS medium supplemented with different concentrations of antibiotics by organogenesis. Results indicated that the regeneration rate of plantlets reached above 90% on MS medium supplemented with 3.0 mg/L BA and 0.3 mg/L NAA. The regeneration shoots were well developed on MS medium supplemented with 0.02 mg/L NAA, 1.0 mg/L KT, and 5.0 mg/L GA3. Among the three kinds of selective antibiotics, the explants are very sensitive to hygromycin and gentamicin, and they will entirely die in two weeks at a concentration of 5 mg/L. However, the shoot differentiation can be inhibited at a concentration of 15 mg/L for kanamycin. Therefore, the optimal antibiotic is kanamycin with an optimum concentration of 15 mg/L. For the two antimicrobial antibiotics, eephamycin has an obviously effect on leaf regeneration. Carbenicillin with a concentration of 250 nag/L can inhibit the development of the agrobacterium strain LBA4404 and affect bud differentiation slightly. Carbenicillin is considered to be the appropriate antimicrobial antibiotic.

关 键 词：矮牵牛 组织培养 遗传转化 再生体系 

分 类 号：S681.6[农业科学—观赏园艺]