金黄色葡萄球菌耐消毒剂基因qacA的研究  

Study on the disinfectant-resistant gene qacA in Staphylococcus aureus

作　　者：王华丽[1] 盛家琦[1] 黄文祥[1] 

机构地区：[1]重庆医科大学附属第一医院重庆市传染病寄生虫病学重点实验室,重庆400016

出　　处：《中国抗生素杂志》2007年第4期229-231,I0001,共4页Chinese Journal of Antibiotics

基　　金：重庆市科委自然基金重点项目

摘　　要：目的检测我院近年来临床分离的金葡菌耐消毒剂的qacA基因水平,为动态监测细菌耐药性的变化趋势、有效控制细菌耐药性的传播提供对策。方法收集我科临床分离的金葡菌共252株,并经本实验室重新鉴定确认。参照美国CLSI/NCCLS标准,用6μg/ml苯唑西林和4%NaCl平板,筛选出耐苯唑西林金葡菌和对苯唑西林敏感的金葡菌。应用PCR基因扩增试验检测金葡菌中耐消毒剂的qacA基因水平。随机抽取一株MRSA阳性株进行基因测序,作qacA基因验证。作qacA基因阳性株的MIC检测,了解阳性株是否有耐药性的表达。结果共检测金葡菌252株,其中MRSA84株(带有qacA基因4株),MSSA168株(带有qacA基因1株)。MRSA阳性菌株对消毒剂的MIC值明显高于MSSA菌株。结论携带含有耐消毒剂基因qacA的金葡菌未在我院流行。Objective To investigate the disinfectant-resistant gene qacA in Staphylococcus aureus isolates from the First Affiliated Hospital of Chongqing Medical University and control drug-resistance transmission of bacteria. Method Two hundred and fifty-two Staphylococcus aureu isolates were collected and reconfirmed by our laboratory. The agar plate containing oxacillin 6μg/ml and 4% NaCl was used to select methicillin-susceptible Staphylococcus aureus and methicillin-resistant Staphylococcous aureus, according to the standard recommended by CLSI/NCCLS （2005） of USA. The qacA gene in the isolates were amplified by PCR. The PCR products were sequenced for verification with the object sequence of GenBank. The drug-susceptible test was performed in qacA carrying stains. Results Among 252 Staphylococcous aureus isolates, only 4 strains were found qacA gene in 84 MRSA and 1 strain in 168. The MICs of antiseptics were higher against MRSA than MSSA. Conclusion Disinfectant-resistant gene qacA -carrying Staphylococcus aureus hasn＇t preriled in the hospital.

关 键 词：金黄色葡萄球菌 消毒剂 qacA基因 

分 类 号：R378.11[医药卫生—病原生物学]