HPLC-MS法测定血浆中氨氯地平的浓度及其药动学研究  

Determination of amlodipine in human plasma with HPLC-MS and its pharmacokinetic study

作　　者：李健华[1] 顾学兰[1] 邹建军[2] 樊宏伟[2] 肖大伟[2] 

机构地区：[1]江苏先声药物研究有限公司,南京210042 [2]南京医科大学附属南京第一医院,南京210006

出　　处：《药学与临床研究》2007年第2期118-121,共4页Pharmaceutical and Clinical Research

摘　　要：目的：建立测定血浆中氨氯地平血药浓度的HPLC—MS法，为临床用药提供参考。方法：血浆中样品用乙酸乙酯提取，HypersilODS柱（4．6mm×150mm，5μm）为分析柱，甲醇-（10mM）醋酸铵水溶液（84：16，v／v）为流动相，氨氯地平的检测离子为[M＋Na]^＋，m／z431．4；内标哌罗匹隆检测离子为[M＋H]^＋，m／z427．5。结果：血浆中氨氯地平的线性范围为0．18～18．2μg·L^-1，批内、批间误差RSD均小于10％，绝对回收率在89．5％-95．2％。结论：本法测定血浆中氨氯地平血药浓度准确、简便，适用于氨氯地平药代动力学的研究。Objective：To establish an analytical method for the determination of amlodipine in human plasma with HPLC -MS, which might provide useful information for clinical practice. Methods： Amlodipine was extracted with ethyl acetate. A Hypersil ODS column was used with mobile phase consisting of methanol-water （containing 10 mmol· L^-1 ammonium acetate） （84： 16, v/v）. The ions for SIR analysis of amlodipine and internal standard perospirone ions were selected at m/z 431.4 and m/z 427.5, respectively. Results .The calibration curve was linear within the range of 0. 18 - 18.2 μg·L^-1. The RSDs of inter-day and intra-day were less than 10% and the recovery was between 89.5% and 95.2%. Conclusions： The method is simple with high sensitivity and good precision and has proved to be suitable for the pharmacokinetic study of amlodipine.

关 键 词：HPLC—MS法 氨氯地平 血药浓度 药代动力学 

分 类 号：R927.2[医药卫生—药学] R969.1