^(60)Co辐射诱变对巴氏蘑菇(姬松茸)子实体多糖组分与结构变化的影响  

Effects of ^(60)Co irradiation on the components and structure of polysaccharide from the fruitbodies of Agaricus blazei Murrill

作　　者：翁伯琦[1] 江枝和[2] 宾文[3] 王义祥[1] 罗涛[2] 林勇[2] 

机构地区：[1]福建省农业科学院农业生态研究所,福州350013 [2]福建省农业科学院食用菌研究开发中心,福州350013 [3]沈阳药科大学制药工程学院,沈阳110016

出　　处：《菌物学报》2007年第1期106-114,共9页Mycosystema

基　　金：福建省科技厅科技项目(2003N047;2005N029)资助

摘　　要：巴氏蘑菇(姬松茸)原菌株J1和辐射突变株J3子实体粗多糖经水提、醇沉、Sevage法去除蛋白,透析得精多糖ABJ-1和ABJ-3;经紫外扫描和凝胶色谱法检测表明,ABJ-1和ABJ-3均具有较高的纯度。以苯酚-硫酸法测得了ABJ-1和ABJ-3的糖含量分别为47%和76.68%,凯氏定氮法测定其含氮量分别为5.77%和21%。,以凝胶过滤法测定了其分子量均在4万左右。完全酸水解后气相色谱分析显示ABJ-1含有甘露糖、葡萄糖、半乳糖等单糖,ABJ-3含有大量的甘露糖及少量的葡萄糖等单糖。红外光谱和Smith降解后气相色谱分析显示ABJ-1和ABJ-3糖基连接键均以a,1-4糖苷键为主。Polysaccharides from the fruitbodies of Agaricus blazei J1 and J3 were extracted and the polysaccharide structure was analyzed. Purified ABJ-1 and ABJ-3 were obtained from crude polysaccharides of Agaricus blazei by water-extracting, alcohol-sedimentation, protein-cleaning（Sevage method） and polysaccharide purification（dialysis method）. Their homogeneity were verified by UV scan and gel chromatography. The carbohydrate content of ABJ-1 and ABJ-3 were 47% and 76.68%, respectively. Their nitrogen contents were 5.77% and 21%, respectively. Their molecular weight were around 40000. The results of gas phase chromatographic analysis after complete acid hydrolysis showed that the composition of ABJ-1 was mannose, glucose, galactose and so on, and the composition of ABJ-3 was mannose and a few glucose. The results of IR spectrum analysis and Smith degradation showed that their glycosyl linkage were mostly α ,1-4.

关 键 词：巴氏蘑菇 子实体 多糖 结构分析 

分 类 号：S646[农业科学—蔬菜学] S567.3[农业科学—园艺学]