二甲双胍对糖尿病大鼠肝脏葡萄糖-6-磷酸酶表达的影响  

Effects of Metformin on Glucose-6-phostapase Expression in Diabetic Rat Liver

作　　者：张景云[1] 张秋梅[1] 于德民[1] 刘德敏[1] 

机构地区：[1]天津医科大学代谢病医院内分泌科,300070

出　　处：《天津医药》2007年第3期197-199,共3页Tianjin Medical Journal

基　　金：天津市自然科学基金资助项目(项目编号:043609211)

摘　　要：目的:探讨二甲双胍抑制肝糖输出,减轻肝脏胰岛素抵抗的分子学机制。方法:建立糖尿病大鼠模型并将之分为正常对照组、糖尿病组、低剂量二甲双胍干预组、高剂量二甲双胍干预组,饲养2周后取出肝脏,以半定量RT-PCR方法测定肝脏葡萄糖-6-磷酸酶(G6Pase)mRNA的表达,蒽酮比色法测定肝脏糖原含量。结果:(1)糖尿病组肝脏G6Pase催化亚基(P36)及转运亚基(P46)mRNA的灰度高于正常对照组(P<0.05),而肝脏糖原含量低于正常对照组(P<0.05)。(2)二甲双胍可以明显减少肝脏G6Pase mRNA表达并增加肝糖原含量(P<0.05),且呈剂量依赖性。结论:二甲双胍能够抑制糖尿病状态下肝脏G6Pase催化亚基及转运亚基mRNA表达,使得6-磷酸葡萄糖向葡萄糖的转化减少,从而减少肝糖输出,达到减轻胰岛素抵抗的作用。Objective： To investigate the mechanism of metformin in hepatic glucose output decreasing and hepatic insulin resistance. Methods： Diabetic model in rats were built in randomly divided into 4 groups including control group, diabetic group, low-dose metformin treated group and high-dose metformin treated group. Livers were taken after 2 weeks treat- ment, G6Pase mRNA expression was measured by reverse transcriptional PCR, and the content of glycogen was measured by anthrone method. Results： （1） The expressions of P36 and P46 mRNA of hepatic G6Pase in diabetic group were significantly higher than those in control group （P 〈 0.05）, and the contents of hepatic glycogen were significantly lower than those in control group （P 〈 0.05）. （2） Metformin decreased the mRNA expression of G6Pase and increased hepatic glycogen significantly（P 〈 0.05）, and the effects were dose dependent. Conclusion： Metformin can inhibit the expression of G6Pase mRNA level, decrease the transformation from 6-phostapase-glucose to glucose, decrease the output of hepatic glucose, and decrease the hepatic insulin resistance.

关 键 词：葡糖-6-磷酸酶 二甲双胍 糖尿病 肝 大鼠 

分 类 号：R587.1[医药卫生—内分泌]