实验性近视眼巩膜成纤维细胞黏弹性研究  

Viscoelastic properties of scleral fibroblasts in experimental myopia

作　　者：陈维毅[1] 王超英[2] 张全有[1] 李阳阳[3] 陈静[1] 董江峰[1] 

机构地区：[1]太原理工大学应用力学与生物医学工程研究所,太原030024 [2]中国人民解放军白求恩国际和平医院眼科,石家庄050082 [3]河北医科大学,石家庄050017

出　　处：《医用生物力学》2007年第1期26-29,58,共5页Journal of Medical Biomechanics

基　　金：国家自然科学基金(N0.102777;N0.10332060)

摘　　要：目的研究豚鼠镜片诱导型近视眼(lens induced myopia,LIM)后极部巩膜成纤维细胞黏弹性特性的变化。方法取2周龄豚鼠10只制备LIM动物模型,对侧眼为自身对照眼,再随机选取10只正常2周龄豚鼠作为正常对照眼。将各组豚鼠后极部巩膜成纤维细胞作体外培养,并传1代,行光镜观察与免疫细胞化学染色法进行细胞鉴定,采用微管吸吮技术表征巩膜成纤维细胞的黏弹性特性。结果经45天凹透镜诱导,实验眼与自身对照眼比较,诱导出(-8．95±0．60)D的相对近视,眼轴延长了(0．60±0．12)mm,两组有显著性差异,P＜0．05;实验组凹透镜诱导前后变化差异有显著意义。凹透镜诱导45天后巩膜成纤维细胞粘弹性测定正常对照组(E_∞=0．30055±0．07713kPa,E_0=0．52553±0．14053kPa,μ=1．94124±1．03281kPa．s,n=52)和自身对照组(E_∞=0．34792±0．09709kPa,E_0=0．59722±0．18118kPa,μ=2．17855±1．22801kPa．s,n=49)均显著低于LIM组(E_∞=0．43555±0．13043kPa,E_0=0．76691±0．21674kPa,μ=4．17255±1．59239kPa·s, n=58)P＜0．05,而正常对照组和自身对照组的各项黏弹性参数差异无显著意义(P＞0．05)。结论凹透镜诱导可以引起明显轴性近视。LIM组后极部巩膜成纤维细胞的黏弹性参数明显高于正常对照组和自身对照组。Objective Using a lens-induced method to establish myopia model in guinea pig to study the changes of viscoelastic properties of sclerotic fibroblasts in the expedmental eye model. Methods 10 two-week-old guinea pigs were lens-induced monocular. A spherical lenses of -10.00D were wear to the unilateral eye with the other eye treated as serf-control. 10 two-week-old guinea pigs were randomly chosen as normal-control. The sclerotic fibroblasts of each group were cultured and passed for I generations in vitro.All this were studied by light microscope and pathology. This study utilized the micropipette aspiration technique with a viscoelastic solid model to investigate the viscoelastic properties of scleral fibroblasts from normal and myopia guinea pigs sclera. Results Eyes became myopic （-9.08±0.21） D and elongated （1.91±0.07） mm 45 days after insertion of the lenses （p〈0.05）. Expedmental studies have shown that the viscoelastic properties of the scleral flbroblasts in LIM group exhibited a significantly higher equilibrium modulus E., instantaneous modulus E0, and apparent viscosity μ （E∞=0.43555±0.13043kPa, E0=0.76691±0.21674kPa, μ=4.17255± 1.59239kPa·s, n=58） compared with normal-control group （E∞=0.30055± 0.07713kPa, E0=0.52553±0.14053kPa, μ= 1.94124±1.03281 kPa·s, n=52, p〈0.05） and self-control group （E∞=0.34792±0.09709kPa, E0= 0.59722±0.18118kPa, μ= 2.17855±1.22801kPa·s, n=49, p〈0.05）. Conclusions Lenses can induce axial growth and lead to myopia in young guinea pigs. The suggest that the scleral fibroblasts become stiffer with the development of myopia.

关 键 词：近视 动物模型 豚鼠 巩膜成纤维细胞 微管吸吮 黏弹性 

分 类 号：Q66[生物学—生物物理学]