鼾症动物模型的建立及其气管力学重建的实验研究  

Construction of an OSAHS animal model and experimental investigation of mechanical remodeling in the tracheae

作　　者：刘永义[1] 覃开蓉[2] 包亚军[1] 陈云俊[1] 刘文华[1] 陈太平[1] 钱兴皋[1] 刘瀛[2] 桑琛[3] 郑明祥[1] 

机构地区：[1]中国人民解放军第359医院耳鼻喉科,镇江212001 [2]上海交通大学力学生物学与医学工程实验室,上海200240 [3]江苏大学生物医学工程研究所,镇江212013

出　　处：《医用生物力学》2007年第1期99-103,共5页Journal of Medical Biomechanics

基　　金：国家自然科学基金(No.10272112)

摘　　要：目的建立阻塞性睡眠呼吸暂停低通气综合征(OSAHS)动物模型,研究咽腔力学环境改变对气管力学特性的影响。方法利用低压舱饲养小型猪获得OSAHS动物模型,测量其气管无载荷、零应力状态的几何尺寸及不同轴向伸长比下的压力(p)—容积(V)关系。进一步计算得到气管的零压顺应性,并与正常对照组比较。结果模型猪出现了明显的类OSAHS表现。与正常对照组相比,模型猪气管无载荷、零应力状态几何尺寸增大,不同伸长比下的零压顺应性增大;模型猪气管黏膜组织的超微结构发生了明显改变。结论提出的建立OSAHS动物模型的方法具有可行性,模型猪气管的形态与结构及力学特性均发生了重建。Objective To set up an obstructive sleep apnea hypopnea syndrome （OSAHS） animal model and investigate the effect of the mechanical environment change in pharyngeal cavity on mechanical property of trachea. Method An OSAHS animal model was obtained by culturing Guizhou mini-type pigs in low pressure chamber;, the in situ longitudinal stretch ratio, the morphologies of no-loed state and zero-stress stateas well as the pressure （p）-volume （V） relationship under different longitudinal stretch ratios of trachea were measured; The zero-pressure compliance describing the mechanical property of trachea was calculated based on the p- V relationship; Compared the obtained data in model pigs with those in control group. Results The OSAHS symptom appeared in the model pigs. Compared with control group, the geometrical sizes of no-load state and zero-stress state in model pigs increases, as same as the zero-pressure compliance under different longitudinal stretch ratios. The ultrastructure of trachea＇s mucosa of the model pig was significantly remodeled. Conclusion The method to establish the OSAHS animal model is reasonable. The geometrical structure and morphology as well as the mechanical property of trachea remodeled have all changed in this mechanical environment of trachea.

关 键 词：阻塞性睡眠呼吸暂停低通气综合征 气管 零应力状态 无载荷状态 p-V关系 零压顺应性 猪 

分 类 号：R318.01[医药卫生—生物医学工程]