杂多酸盐K_7[PTi_2W_(10)O_(40)]·6H_2O与牛血清白蛋白相互作用的研究  

Interaction between Heteropoly Salt K_7[PTi_2W_(10)O_(40)]·6H_2O and Bovine Serum Albumin

作　　者：梁彦秋[1,2] 邓斌[3] 刘婷婷[3] 孙鹏[3] 臧树良[1] 

机构地区：[1]华东师范大学化学系 [2]沈阳化工学院环境与生物系,沈阳110142 [3]辽宁大学化学院

出　　处：《无机化学学报》2007年第4期688-692,共5页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金资助项目(No.20271024)

摘　　要：20世纪70年代初期。法国科学家报道了杂多阴离子[SiW12O40]^4-的抗病毒活性，以后相继发现了[BW12O40]^5-[P2W12O40]^6-等在体外也表现出抗病毒活性。80年代后期。具有抑制艾滋病病毒作用的（NH4）17Na[NaSb9W21O86]·14H2O（HPA-23）被应用于临床试验回。虽然其治疗效果不够理想．但却激发了人们对多金属氧酸盐的研究兴趣。90年代以来．Yamase等研究了十几种多金属氧酸盐的抗肿瘤、抗爱滋病毒活性，发现K7[PTi2W10O40]·6H2O（PM-19）具有较强的抗HIV病毒能力和较低的毒性。此后多金属氧酸盐的药物化学迅速发展起来。Under the imitated physiological condition of animal body, the interactions of heteropoly salt （PM-19） with bovine serum albumin （BSA） were investigated by fluorescence and absorption spectroscopy. It was shown that this compound had a quite strong ability to quench the fluorescence launching from BSA. After analyzing the fluorescence quenching data according to Stern-Volmer equation and Lineweaver-Burk double-reciprocal equation, we found that BSA had reacted with PM-19 and formed a certain new compound. The quenching belonged to static fluorescence quenching. According to Lineweaver-Burk equation, the forming constants of the compound （298 K： 2.68 × 10^5 L ·mol^-1; 304 K： 2.19 × 10^5 L ·mol^-1; 310 K： 1.82 × 10^5 L ·mol^-1） and the thermodynamic parameters （△H=-24.72 k J·mol^-1; △S=20.97 J·mol^-1·K^-1 / 20.92 J·mol^-1·K^-1 / 20.97 J·mol^-1·K^-1; △G=-30.97 kJ·mol^-1 / -31.08 kJ· mol^-1 / -31.22kJ· mol^-1） at the corresponding temperatures were obtained. The latter shows that binding power between them is mainly electrostatic interaction. Based on Foerster＇s non-radiation energy transfer mechanism, the binding locality （r=4.14 nm） was calculated between donor and accepter. The effect of PM-19 on the conformation of BSA was also analyzed by synchronous fluorescence spectroscopy.

关 键 词：杂多酸盐 牛血清白蛋白 荧光光谱 紫外光谱 热力学参数 同步荧光光谱 

分 类 号：O657.39[理学—分析化学]