自体移植皮片中α-黑素细胞刺激素的表达与黑色素含量的相关性  

Correlation between expression of α-melanocyte-stimulating hormone and melanin level in human skin autografts

作　　者：薛春雨[1] 邢新[1] 李蠡[1] 李军辉[1] 张敬德[1] 蒋平[2] 龚志锦[3] 

机构地区：[1]第二军医大学长海医院整形外科,上海200433 [2]第二军医大学基础部生物化学与分子生物学教研室,上海200433 [3]第二军医大学病理学教研室

出　　处：《第二军医大学学报》2007年第3期302-305,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30371473).~~

摘　　要：目的:检测α-黑素细胞刺激素(α-melanocyte sti mulating hormone,α-MSH)及黑色素在不同类型自体移植皮片中的表达,并与自身正常皮肤作对照,初步认识α-MSH在自体移植皮片过度色素沉着中的作用。方法:利用免疫组织化学方法和银染-丽春红法检测自体移植皮片(包括全厚皮片、中厚皮片及刃厚皮片)与原供区及受区周围自体正常对照皮肤中α-MSH的蛋白表达及表皮中黑色素的含量。结果:α-MSH的表达定位于表皮基底部黑素细胞、角质形成细胞胞质,在大部分自体移植皮片中呈阳性表达,皮片愈薄α-MSH的表达愈明显(P<0.01);α-MSH在各类型皮片中的表达显著高于在自体正常对照皮肤中的表达(P<0.01);α-MSH在受区周围与原供区正常对照皮肤中的表达无显著性差异。不同类型皮片表皮中黑色素的含量较自体正常对照皮肤中黑色素含量明显增加(P<0.01);不同类型皮片间表皮中黑色素的含量亦均有显著性差异(P<0.01),皮片愈薄黑色素的含量愈多,且皮片中黑色素的含量与α-MSH的表达量呈正相关。结论:自体移植皮片中黑色素的含量与α-MSH的表达量呈正相关,表明α-MSH在自体移植皮片中的高表达能使皮片中黑色素含量增加,在自体移植皮片过度色素沉着中起重要调控作用。Objective：To examine the expression of α-melanocyte-stimulating hormone（α-MSH） and the melanin in different kinds of human skin autografts and in the normal skins, so as to elucidate the role of α-MSH in hyperpigmentation in the skin autografts. Methods： Immunohistochemical technique was employed to detect the expression and distribution of α-MSH in skin autografts （including the full thickness skin autografts, medium thickness skin autografts, razor-thin skin autografts, and normal skins adjacent to the donor site and the recipient site）. Masson-Fontana staining technique was used to detect the melanin contents in all the above skin specimens. Results： The location of α-MSH expression was at the cytoplasm of melanocytes and keratinocytes in epiderm; α-MSH was positive in most skin autografts and its expression was higher in the thinner skin autografts. The expression of α-MSH in all types of skin autografts was significantly different from that in normal skin （P〈 0.01） ; α-MS expression was also significantly different between all the skin autografts （P 〈 0. 01） ; α-MSH expression in normal skin around donor site and recipient site autografts was obvious increased compared with that in normal had no statistical difference. The contents of melanin in skin skin（P〈0.01） ; the contents of melanin between all the skin autografts were also significantly different （P〈0.01）. The melanin contents increased with the decrease of skin autografts thickness. The expression of α-MSH was positively correlated with the contents of melanin in epidermis. Conclusion： The expression of α-MSH in skin autografts is positively correlated with the contents of melanin in skin autografts. Overexpression of α-MSH may play an important role in hyperpigmentation process of skin autografts.

关 键 词：α促黑素 皮肤移植 移植 自体 色素沉着过多 黑素类 

分 类 号：R622.1[医药卫生—整形外科]