变异型IκBα抑制人类多形性胶质母细胞瘤细胞中血管生长因子VEGF及IL-8的表达  

Mutant-type IκBα inhibit the expression of VEGF and IL-8 in human glioblastoma multiform cells

在线阅读下载全文

作  者:吴建梁[1] 扈玉华[1] 史学芳[1] 马树成[1] 

机构地区:[1]河北医科大学第二医院神经外科,石家庄050000

出  处:《中华神经外科杂志》2007年第3期224-226,共3页Chinese Journal of Neurosurgery

基  金:国家自然科学基金项目(30440016);河北省卫生厅科研基金项目(06135)

摘  要:目的探讨变异型IκBα(IκBαM)基因对人类多形性胶质母细胞瘤(Glioblastoma multiform,GBM)的细胞生长及其内血管内皮成长因子(VEGF)、白细胞介素-8(IL-8)表达的调控作用。方法通过构建质粒、基因转染以及IκBαM蛋白表达的筛选,建立稳定表达IκBαM的人类GBM细胞株,进而检测转染后细胞的体外生长曲线,并运用Northern blot技术分析细胞内VEGF、IL-8在RNA水平的表达。结果用Western blot法成功筛选了转染的阳性细胞克隆,并发现转染后的人类GBM细胞与对照相比,体外生长曲线无明显差异,但其中VEGF、IL-8在RNA水平的表达量显著降低。结论IκBαM基因对人类GBM细胞中的血管生长因子VEGF、IL-8的表达具有抑制作用。Objective To investigate the effect of mutant-type IκBα(IκBαM) on the growth of human glioblastoma multiform(GBM) cell lines and the expression of VEGF and IL-8. Methods Human GBM cell lines were used, transfected with IκBαM gene and the cell growth rate were examined. Then total RNA were isolated and the expression of VEGF and IL-8 were analyzed by Northern blot. Results Expression of exogenous wild-type and mutant-type IκBα were verified by Western blot analysis. No significant difference in the in vitro growth rate of the cell lines were observed, but the expression of VEGF and IL-8 in RNA level were decrease in the IκBαM treated cells. Conclusion IκBαM can inhibit the expression of VEGF and IL-8 in human GBM cell lines.

关 键 词:变异性IκBα 多形性胶质母细胞瘤 血管内皮生长因子 白细胞介素-8 表皮生长因子受体 

分 类 号:R686[医药卫生—骨科学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象