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作 者:吴建梁[1] 扈玉华[1] 史学芳[1] 马树成[1]
机构地区:[1]河北医科大学第二医院神经外科,石家庄050000
出 处:《中华神经外科杂志》2007年第3期224-226,共3页Chinese Journal of Neurosurgery
基 金:国家自然科学基金项目(30440016);河北省卫生厅科研基金项目(06135)
摘 要:目的探讨变异型IκBα(IκBαM)基因对人类多形性胶质母细胞瘤(Glioblastoma multiform,GBM)的细胞生长及其内血管内皮成长因子(VEGF)、白细胞介素-8(IL-8)表达的调控作用。方法通过构建质粒、基因转染以及IκBαM蛋白表达的筛选,建立稳定表达IκBαM的人类GBM细胞株,进而检测转染后细胞的体外生长曲线,并运用Northern blot技术分析细胞内VEGF、IL-8在RNA水平的表达。结果用Western blot法成功筛选了转染的阳性细胞克隆,并发现转染后的人类GBM细胞与对照相比,体外生长曲线无明显差异,但其中VEGF、IL-8在RNA水平的表达量显著降低。结论IκBαM基因对人类GBM细胞中的血管生长因子VEGF、IL-8的表达具有抑制作用。Objective To investigate the effect of mutant-type IκBα(IκBαM) on the growth of human glioblastoma multiform(GBM) cell lines and the expression of VEGF and IL-8. Methods Human GBM cell lines were used, transfected with IκBαM gene and the cell growth rate were examined. Then total RNA were isolated and the expression of VEGF and IL-8 were analyzed by Northern blot. Results Expression of exogenous wild-type and mutant-type IκBα were verified by Western blot analysis. No significant difference in the in vitro growth rate of the cell lines were observed, but the expression of VEGF and IL-8 in RNA level were decrease in the IκBαM treated cells. Conclusion IκBαM can inhibit the expression of VEGF and IL-8 in human GBM cell lines.
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