圆盘状受体在病理性瘢痕成纤维细胞中表达  

Discoidin domain receptors（DDRs）expresssion in pathological scar fibroblast formation

作　　者：姜育智[1] 邢新[1] 文军慧[2] 薛春雨[1] 张敬德[1] 张明利[1] 

机构地区：[1]上海长海医院整形外科,200433 [2]上海长海医院中心实验室,200433

出　　处：《中华整形外科杂志》2007年第2期134-136,共3页Chinese Journal of Plastic Surgery

摘　　要：目的 探讨圆盘状受体（discoidin domain receptors，DDRs）在病理性瘢痕形成中的作用。方法 取13例瘢痕疙瘩，10例增生性瘢痕，10例正常皮肤，体外培养成纤维细胞，经荧光定量PCR，定量检测成纤维细胞中DDR1、DDR2水平。结果 瘢痕疙瘩、增生性瘢痕成纤维细胞中DDR1水平明显高于正常皮肤成纤维细胞（20．98对4．2，P〈0．01；7．9对4．23，P〈0．05），瘢痕疙瘩与增生性瘢痕成纤维细胞中DDR1相比，有统计学意义（20．98对7．9，P〈0．01），DDR2在瘢痕疙瘩、增生性瘢痕成纤维细胞和正常皮肤成纤维细胞中差异无统计学意义（358，332对278，P〉0．05）。结论 荧光定量PCR可准确测定组织中DDR含量，病理性瘢痕成纤维细胞的细胞学行为与DDR有关。Objective To explore the role of diseoidin domain reeeptors（DDRs）in the formation of the keloid. Methods The real-time quantitative PCR was used to compare the DDRs expression in the keloids and normal fibroblasts. Results The level of DDR1 expression was significantly higher in keloid than in normal fibroblast（20.98 vs 4.2, P 〈 0.01 ; 7.9 vs 4.23,P 〈 0.05）. The level of DDR1 expression in keloid was also higher significantly than that in hypertropic scar（20.98 vs 7.9, P 〈0.01）. However, the level of DDR2 expression was somewhat higher in keloid than in normal fibroblasts, the difference seemed not to be significantly in probability （358, 332 vs 278, P〉0.05）. Conclusions DDRs may exert effect on keloid cell behaviours.

关 键 词：圆盘状受体 荧光定量PCR 瘢痕疙瘩 增生性瘢痕 

分 类 号：R686[医药卫生—骨科学]