表皮葡萄球菌全面调节子的表达在生物被膜耐药性中的作用  

Relationship between expressions of global regulators and erythromycin resistance of Staphylococcus epidermidis biofilm

作　　者：贾宁[1] 徐志凯[2] 袁云娥[1] 索继江[1] 邢玉斌[1] 

机构地区：[1]解放军总医院感染管理与疾病控制科,北京100853 [2]第四军医大学基础部微生物学教研室,陕西西安710033

出　　处：《第四军医大学学报》2007年第7期600-602,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30300296)

摘　　要：目的:探讨表皮葡萄球菌全面调节子的表达在生物被膜耐药性中的作用.方法:采用实时荧光定量RT-PCR对表皮葡葡球菌1457生物被膜内细菌红霉素作用前后全面调节子(agr,sarA,sigB)的表达进行检测.结果:在表皮葡萄球菌1457中,agrD和RNAⅢ在膜内菌中的表达均高于浮游菌,且红霉素作用24h后的表达高于作用前(P<0.05);sarA膜内菌的表达高于浮游菌(P<0.05),但红霉素作用前后的表达无统计学差异(P>0.05);膜内菌sigB和反应sigB调控的活性基因asp23的表达红霉素作用前后无统计学差异(P>0.05).结论:由于agrD和RNAⅢ参与群体效应系统的调节,提示群体效应调节在红霉素耐药性增加中起着重要作用.AIM： To explore the relationship between the expressions of global regulators and the erythromycin resistance of Staphylococcus epidermidis （ S. epidermidis ） biofilm. METHODS ： The expressions of global regulators （ agr, sarA and sigB） in biofilm cells of S. epidermidis 1457 with and without erythromycin resistance were detected by SYBR green Ⅰ real-time RT-PCR. RESULTS： The expressions of agrD and RNAⅢ were significantly higher in biofilm cells than those in planktonic cells in both erythromycin and non-erythromycin groups （ P 〈 0.05 ）. The expression of sarA in biofilm cells was significantly higher than that in planktonic cells, but there was no significant difference in biofilm cells between erythromycin group and nonerythromycin group. The expression of sigB and its active regulation gene asp23 in biofilm cells showed no significant difference between erythromycin and non-erythromycin groups （ P 〉 0.05 ）. CONCLUSION： agrD and RNAIII take part in quorum-sensing regulation, which suggests that quorum-sensing system plays an important role in erythromycin resistance of S. epidermidis biofilm.

关 键 词：表皮葡萄球菌 生物膜 实时定量 逆转录聚合酶链反应 

分 类 号：R515.9[医药卫生—内科学]