阿托伐他汀对人肾小球系膜细胞增殖、TGF-β1 mRNA和p38MAPK表达的影响  

EFFECT OF ATORVASTATIN ON CELL PROLIFERATION,TGF-β1 mRNA AND p38MAPK EXPRESSION

作　　者：李萍[1] 张海燕[2] 刘国良[2] 盛志业[1] 马小峰[3] 向军[4] 

机构地区：[1]沈阳军区总医院内分泌科 [2]中国医科大学附属第一医院内分泌科,沈阳110001 [3]沈阳军区总医院呼吸内科 [4]沈阳军区总医院泌尿外科,沈阳110001

出　　处：《中国组织化学与细胞化学杂志》2007年第1期57-61,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：辽宁省重点科技攻关项目(2001225004)

摘　　要：目的观察阿托伐他汀(atorv)对氧化低密度脂蛋白(ox-LDL)诱导的人肾小球系膜细胞(HGMCs)增殖和转化生长因子β1(TGF-β1)mRNA及丝裂原活化蛋白激酶(p38MAPK)蛋白表达的影响。方法在体外培养HG-MCs,用MTT法检测细胞增殖,用半定量RT-PCR法检测细胞TGF-β1 mRNA表达,用Western blot法检测细胞p38MAPK蛋白合成。结果1.Ox-LDL(80μg/ml)刺激系膜细胞增殖;2.Ox-LDL(10μg/ml-80μg/ml)以浓度依赖的方式增加HGMCs TGF-β1 mRNA和p38MAPK蛋白表达,3.Atovastatin(6μg-12μg/ml)抑制系膜细胞增殖,降低ox-LDL引起的TGF-β1 mRNA表达上调,抑制p38MAPK信号途径激活。结论阿托伐他汀可能通过对抗p38MAPK信号通路,减少TGFβ1分泌,抑制ox-LDL引起的肾小球系膜细胞增殖,预防和治疗伴有血脂异常的糖尿病肾脏病变。Objective 1. To observe the effect of atorvastatin on cell proliferation and expression of TGF-β1 mRNA and p38MAPK in human glomerular mesangial cells （HGMCs） induced by ox-LDL. Methods Cultured human glomerular mesangial cells were used. Cell proliferation was measured by MTT, TGF-β1 mRNA expression by RT-PCR, and p38MAPK protein expression in HGMCs by Western blot. Results 1. Ox-LDL （80μg/ml） stimulated mesangial cell proliferation; 2. Ox-LDL （10μg/ml-80μg/ ml） increased the expression of HGMCs TGF-β1 rnRNA in a concentration-dependent manner; 3. Atorvastatin （6μg/ml- 12μg/ml） inhibited mesangial cell proliferation and attenuated TGFβ1 mRNA and p38MAPK expression upregulation induced by Ox-LDL. Conclusion Atorvastatin may exert its beneficial effect by inhibiting mesangial cell proliferation and attenuating TGF-β1 mRNA and p38MAPK expressions in diabetic nephropathy.

关 键 词：阿托伐他汀 细胞增殖 转化生长因子Β1 丝裂原活化蛋白激酶 人肾小球系膜细胞 

分 类 号：R587.1[医药卫生—内分泌]