猪和野生动物源大肠杆菌及沙门菌中磺胺类药物耐药基因的检测  

Detection of sulfonamides-resistant genes in Salmonella and Escherichia coli isolated from pigs and wild animals

作　　者：周万蓉[1] 王红宁[2] 张安云[1] 吴琦[2] 黄勇[2] 柳萍[2] 田浪[2] 杨鑫[2] 刘立[2] 夏青青[2] 

机构地区：[1]四川农业大学资源环境学院,四川雅安625014 [2]四川农业大学动物科技学院

出　　处：《中国兽医科学》2007年第4期287-290,共4页Chinese Veterinary Science

基　　金：国家"十五"重点科技攻关项目(2002BA514A-12-5);国家农业科技成果转化项目(05EFN215100243)

摘　　要：对从四川省10个规模化猪场和16种野生动物的粪样中分离鉴定出的67株大肠杆菌、57株沙门菌进行了药敏试验。结果,猪源和野生动物源分离菌对磺胺类药物的耐药率分别为72.0%(72/100)和29.2%(7/24)。根据GenBank中登录的序列,设计了3对特异性引物,对磺胺类药物的耐药基因Sul1、Sul2、Sul3进行了三重PCR检测。在这124株细菌中,Sul1基因的检出率最高,为47.6%(59/124),Sul2基因的检出率为17.7%(22/124),Sul3基因的检出率为18.5%(23/124)。药敏试验结果与基因检测结果的符合率为89.9%。A drug sensitivity test was carried out in 50 Escherichia coli strains and 50 Salmonella strains isolated from pigs on large-scale pig farms and 17 E. coli strains and 7 Salmonella strains isolated from 16 species of wild animals in Sichuan Province. The sulfonamides resistance rates of the isolates from pigs and wild animals were 72.0% （72/100） and 29.2% （7/24）, respectively. Sulfonamides-resistant genes Sul1,Sul2 and Sul3 were detected by the multiplex-PCR with 3 pairs of primers designed according to the sequences available in GenBank. The detection rates of the sulfonamides-resistant genes Sul1, Sul2 and Sul3 were 47.6% （59/124）, 17.7% （22/124）, 18.5 % （23/124）, respectively. The consistency rate between results of the drug sensitivity test and that of the multiplex-PCR was 89.9 %.

关 键 词：猪 野生动物 大肠杆菌 沙门菌 磺胺类药物 耐药基因 

分 类 号：S852.61[农业科学—基础兽医学] Q503[农业科学—兽医学]