维生素C对宫颈癌HeLa细胞增生和细胞凋亡的影响  

The Effects of Vitamin C on the Growth and Apoptosis of Hela Cell

作　　者：王艳华[1] 黄利鸣[2] 

机构地区：[1]三峡大学医学院生物研究所,宜昌443002 [2]三峡大学医学院病理教研室,宜昌443002

出　　处：《医学研究杂志》2007年第4期44-46,共3页Journal of Medical Research

摘　　要：目的探讨维生素C在体外对人宫颈癌HeLa细胞的增生和凋亡的影响,以促进维生素C在肿瘤治疗上的应用。方法倒置显微镜下观察其形态学改变;MTT法检测维生素C对HeLa细胞增生的影响;流式细胞仪分析细胞周期和凋亡率;琼脂糖凝胶电泳分析凋亡过程中细胞基因组DNA的变化。结果维生素C以浓度和时间依赖的方式阻滞HeLa细胞于G0/G1期,抑制其生长并诱导凋亡。结论维生素C可抑制HeLa细胞生长,使细胞阻滞于G0/G1期并诱导细胞凋亡。其原因可能是HPVE6蛋白的表达上调而恢复了P53的功能。Objective To discuss the effects of vitamin C in vitro,on human cancer HeLa cell multiplication and apoptosis, in order to promote vitamin C in the tumor treatment application. Methods the morphology changeof cervical cancer Hela cell was observed under the inverted microscope ; the effects of vitamin e on Hela cell by MTT assay ; cell cycle and weakly perishing rate was analysed rate was analysed by Flow CytoMeter;applying the agarose gelatin electricity swims to analyse weakly perishes in the process the cell gene group DNA change. Results Vitamin C hinders the HeLa cell by the density and the time dependence way to the G0/G1 period, suppresses it to grow and to induce weakly perishes. Conclusions Vitamin C may suppress the HeLa cell to grow, causes the cell to hinder to the G0/G1 period and induces cell weakly perishing. Its reason possibly was HPV E6 protein expression surrenders to restoring the function of P53 gene.

关 键 词：宫颈肿瘤 维生素类 乳头瘤病毒 

分 类 号：R284.1[医药卫生—中药学]