农杆菌介导的薰衣草愈伤组织遗传转化  

Agrobacterium-mediated Transformation of Lavandula angustifolia

作　　者：程娜[1] 姚大年[1] 张文明[1] 王晓军[2] 赵民安[2] 

机构地区：[1]安徽农业大学农学院,安徽合肥230036 [2]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011

出　　处：《安徽农业科学》2007年第11期3181-3182,3226,共3页Journal of Anhui Agricultural Sciences

摘　　要：选用带有天山雪莲冷诱导蛋白基因的载体pCAMBIA1304,通过根癌农杆菌EHA105介导,将其转到薰衣草愈伤组织中,经潮霉素筛选,获得抗性愈伤组织,抗性愈伤率为35.3%。经GFP瞬时检测和PCR检测,表明外源基因已经整合到薰衣草基因组中,从而有可能提高薰衣草的抗寒性。A transformation system for Lavandula angustifolia was developed using Agrobacterium tumefaciens-mediated gene delivery system. Leaf induced callus was used as explants. The binary vector pCAMBIA1304 which contained GFP and cold -inducible protein gene was introduced into the Agrobacterium strain EHA105 and then transformed into callus. Resistant callus was selected by hygromycin. The resistant percentage of callus was 35.3 %. GFP gene expression and PCR analysis result confirmed that the cold-inducible protein gene was integrated successfully into Lavandula angustifolia genome, which might improve the cold resistance of Lavandula angustifolia.

关 键 词：薰衣草 愈伤组织 冷诱导蛋白 转化 

分 类 号：Q813.1[生物学—生物工程]