针药结合对乳腺增生大鼠乳腺组织及雌激素受体亚型表达的影响  

Effects of combined acupuncture and medicine therapy on the breast tissue and expressions of estrogen receptor subgroups in the rat of hyperplasia of mammary glands

作　　者：刘丽军[1] 杜惠兰[1] 靳亚慈[1] 陈景伟[1] 

机构地区：[1]河北医科大学中西医结合学院

出　　处：《中国针灸》2007年第4期279-283,共5页Chinese Acupuncture & Moxibustion

摘　　要：目的:研究针药结合对大鼠乳腺增生病模型的抑制作用及作用机制。方法:复制乳腺增生病模型,造模后随机分为针刺组、中药组、针药组、对照组、模型组,除正常组、模型组外,其他治疗组分别行针刺、中药、针药结合及西药三苯氧胺治疗。每日治疗1次,9次一疗程,治疗3个疗程(30天)。观察乳腺组织的形态变化,测定腺泡腔直径及面积,免疫组化检测雌激素受体亚型(ERα、ERβ)的表达。结果:模型组腺泡腔直径及面积均较正常组增大(均P<0.01);治疗组腺泡腔直径及面积均较模型组减小(P<0.05,P<0.01);针刺和中药可上调ERβ、下调ERα表达。结论:针刺和中药均对大鼠乳腺增生有抑制作用,针药结合的抑制作用最强,基本接近三苯氧胺水平,其治疗机制可能与上调ERβ、下调ERα表达有关。Objective To study on the inhibitory action of combined acupuncture and medicine therapy on the model rat of hyperplasia of mammary glands and the mechanism. Methods The model rat of hyperplasia of mammary glands were prepared. After modelling, they were randomly divided into an acupuncture group, a Chinese drug group and a combined acupuncture and drug group, a control group and a model group. Except both the control group and the model group, other 4 groups were treated respectively with acupuncture, Chinese drug, combined acupuncture and Chinese drug, and Premormine, once each day, 9 sessions constituting one course. After treatment of 3 courses （30 days）, changes of the breast tissue form were observed, and the diameter and the area of the acina cavity were determined and expressions of estrogen receptor subgroups （ERα and ERβ） were detected with immunohistochamical methods. Results The diameter and the area of the acina cavity were increased in the model group as compared with those in the normal group （both P〈0. 01）, and in the treatment group they were decreased as compared with those in the model group （P〈0.05 or P〈0. 01）） ; both acupuncture and Chinese drug could up-regulate the expression of ERβ and down-regulate the expression of ERα. Conclusion Both acupuncture and moxibustion, and Chinese medicine have inhibitory action on hyperplasia of mammary glands in the rat, with the strongest inhibitory action of the combined acupuncture and medicine treatment which is basically close to the level of Premormine. The mechanism is possily related with the up-regulation of ERβ expression and down-regulation of ERα expression.

关 键 词：针药并用 纤维囊性病 乳腺/针灸疗法 纤维囊性病 乳腺/中医药疗法 基因表达 乳房/ 代谢 乳房/病理学 

分 类 号：R246.3[医药卫生—针灸推拿学]